Improved phytoremediation of oilseed rape (Brassica napus) by Trichoderma mutant constructed by restriction enzyme-mediated integration (REMI) in cadmium polluted soil
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Abstract
In this study, oilseed rape (Brassica napus) was exploited in remediation of Cd-contaminated
soil in combination of Trichodermakoningii. To improve its phytoextracting efficiency,
restriction enzyme-mediated integration was used to construct Trichoderma mutants
with higher Cd resistance. Of 200 mutants, 10 mutants were shown with higher Cd tolerance
and enhanced ability of removing Cd from growth medium. In pot experiment, mutant
P6 significantly alleviated the negative impacts of Cd on oilseed rape growth, and
improved the Cd uptake ability of oilseed rape shoot in Cd contaminated soil (p<0.05).
Based on the dry weight, the amounts of Cd in shoots of mutant P6 treated oilseed
rape were increased by 23% and 38% per pot compared with wild type Trichoderma treatment;
53% and 107% against non-inoculated treatment, respectively, at 20 and 50mgCdkg(-1)
soil. The results suggested the Trichoderma mutant-oilseed rape symbiosis system could
be used in remediation of soil contaminated with heavy metal Cd.