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      Berberine inhibits the LPS-induced proliferation and inflammatory response of stromal cells of adenomyosis tissues mediated by the LPS/TLR4 signaling pathway

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          Abstract

          A previous study by our group has demonstrated that lipopolysaccharide (LPS) induces adenomyosis through stimulating inflammatory cell proliferation and invasive growth of stromal cells via Toll-like receptor 4 (TLR4) signaling. The present study aimed to investigate the effects of berberine (BBR) on LPS-induced ectopic endometrial stromal cells (EESCs) isolated from patients with adenomyosis. The viability of EESCs treated with LPS or LPS plus BBR was detected by a cell counting kit-8 assay, and the cell cycle distribution and apoptosis were evaluated by flow cytometry. The effect of BBR on the expression of key molecules of inflammatory proliferation and invasive growth of LPS-induced EESCs was also evaluated. BBR significantly inhibited the LPS-induced proliferation of EESCs in a dose- and time-dependent manner. BBR induced cell cycle arrest in G0/G1 phase and enhanced apoptosis of LPS-induced EESCs. Furthermore, BBR inhibited the expression of interleukin (IL)-6, IL-8, transforming growth factor-β, epithelial growth factor, vascular endothelial growth factor and matrix metalloproteinase 2 in LPS-induced EESCs. To the best of our knowledge, the present study was the first to demonstrate that BBR has a protective effect on ameliorating the LPS-induced progression of adenomyosis. This result may provide a novel therapeutic strategy for the clinical treatment of the disease.

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          Author and article information

          Journal
          Exp Ther Med
          Exp Ther Med
          ETM
          Experimental and Therapeutic Medicine
          D.A. Spandidos
          1792-0981
          1792-1015
          December 2017
          16 October 2017
          16 October 2017
          : 14
          : 6
          : 6125-6130
          Affiliations
          Department of Obstetrics and Gynecology, Yangpu Hospital, Tongji University School of Medicine, Shanghai 200090, P.R. China
          Author notes
          Correspondence to: Dr Zhongping Cheng, Department of Obstetrics and Gynecology, Yangpu Hospital, Tongji University School of Medicine, 450 Tengyue Road, Shanghai 200090, P.R. China, E-mail: mdcheng18@ 123456263.net
          Article
          PMC5740511 PMC5740511 5740511 ETM-0-0-5316
          10.3892/etm.2017.5316
          5740511
          29285168
          f434fdf1-a56a-4f80-a3a8-5cf162f8c221
          Copyright © 2017, Spandidos Publications
          History
          : 24 March 2017
          : 15 August 2017
          Categories
          Articles

          lipopolysaccharide,proliferation,stromal cells,adenomyosis,berberine

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