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      Graphitized carbon nanofiber-Pt nanoparticle hybrids as sensitive tool for preparation of screen printing biosensors. Detection of lactate in wines and ciders.

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          Abstract

          This work describes the fabrication of a new lactate biosensor. The strategy is based on the use of a novel hybrid nanomaterial for amperometric biosensors i.e. platinum nanoparticles (PtNps) supported on graphitized carbon nanofibers (PtNps/GCNF) prepared by chemical reduction of the Pt precursor at GCNF surfaces. The biosensors were constructed by covalent immobilization of lactate oxidase (LOx) onto screen printed carbon electrodes (SPCEs) modified with PtNps (PtNps/GCNF-SPCEs) using polyethyleneimine (PEI) and glutaraldehyde (GA). Experimental variables concerning both the biosensor design and the detection process were investigated for an optimal analytical performance. Lactate biosensors show good reproducibility (RSD 4.9%, n=10) and sensitivity (41,302±546) μA/Mcm(2), with a good limit of detection (6.9μM). Covalent immobilization of the enzyme allows the reuse of the biosensor for several measurements, converting them in a cheap alternative to the solid electrodes. The long-term stability of the biosensors was also evaluated. 90% of the signal was kept after 3months of storage at room temperature (RT), while 95% was retained after 18months at -20°C. These results demonstrate that the method provides sensitive electrochemical lactate biosensors where the stability of the enzymatic activity can be preserved for a long period of time in adequate storage conditions.

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          Author and article information

          Journal
          Bioelectrochemistry
          Bioelectrochemistry (Amsterdam, Netherlands)
          Elsevier BV
          1878-562X
          1567-5394
          Feb 2015
          : 101
          Affiliations
          [1 ] Sensors Unit, Materials Division, IK4-CIDETEC, Parque Tecnológico de San Sebastián, 20009 Donostia-San Sebastián, Spain. Electronic address: oloaiza@cidetec.es.
          [2 ] Sensors Unit, Materials Division, IK4-CIDETEC, Parque Tecnológico de San Sebastián, 20009 Donostia-San Sebastián, Spain.
          [3 ] Department of Applied Physics, Aalto University School of Science, P.O. Box 15100, 00076 Aalto, Finland.
          Article
          S1567-5394(14)00111-X
          10.1016/j.bioelechem.2014.07.005
          25108199
          f4567a27-531c-4dd4-9a7a-12107bd67756
          History

          Screen printing electrodes,Beverages,GCNF,Lactate biosensor,PtNps

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