Analysis of eleven iridoid glycosides by micellar electrokinetic capillary chromatography (MECC) and screening of plant samples by partial filling (MECC)–electrospray ionisation mass spectrometry
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Abstract
Of ammonium, lithium and sodium salts of dodecyl sulfate studied as surfactants in
the separation of iridoid glycosides by micellar electrokinetic capillary chromatography
(MECC), the last one gave the best results. Eleven neutral iridoid glycosides were
separated by MECC with sodium dodecyl sulfate as surfactant, and the water-micelle
partition coefficients of the compounds were calculated. The separation system was
coupled via a coaxial sheath flow electrospray interface to a mass spectrometer, and
the partial filling technique was used in the on-line analysis. Seven plant species
belonging to five genera (Plantago, Veronica, Melampyrum, Succisa and Valeriana) were
screened for the iridoid glycosides by the new method that was developed. The findings
confirmed those of an earlier study on five of the iridoid glycosides. Some new iridoid
glycosides were found in Plantago lanceolata, Veronica spicata and V. chamaedrys.