First molecular detection of tick-borne pathogens in dogs from Jiangxi,
China

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Abstract

In this study, blood samples obtained from 162 dogs in Jiangxi, China, were employed in molecular screening of canine tick-borne pathogens by PCR and sequencing. Babesia spp. gene fragment was detected in 12 (7.41%) dogs. All samples were negative for Hepatozoon spp., Ehrlichia canis, Coxiella spp., Borrelia spp., Rickettsia spp. and Anaplasma platys. Species-specific PCR analysis further confirmed that 8 (4.94%) and 4 (2.47%) dogs were infected by Babesia canis vogeli and Babesia gibsoni, respectively. Based on our analyses, Babesia spp. infection in Jiangxi appeared not related to age, gender, breed, usage, activity and health status or tick infestation history of the dogs. This is the first molecular report of Babesia canis vogeli and Babesia gibsoni in dogs from Jiangxi, China.

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Most cited references 23

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Tick-borne infectious diseases of dogs.

Susan E. Shaw, Michael J Day, Richard Birtles … (2001)

Tick-transmitted infections are an emerging problem in dogs. In addition to causing serious disease in traditional tropical and semi-tropical regions, they are now increasingly recognized as a cause of disease in dogs in temperate climates and urban environments. Furthermore, subclinically infected companion animals could provide a reservoir for human tick-transmitted infectious agents, such as Ehrlichia chaffeensis, Ehrlichia ewingll, the Ehrlichia phagocytophila group and Rickettsia conorii. Here, we discuss the emergence of new canine tick-transmitted diseases, which results from several factors, including the expansion of the tick range into urban and semi-urban areas worldwide, the movement of infected dogs into previously non-endemic areas, and the advent of novel molecular techniques for diagnosis and pathogen identification.

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Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of São Paulo, Brazil, where Brazilian spotted fever is endemic.

Marcelo B Labruna, Ted Whitworth, Mauricio C Horta … (2003)

Owing to the potential role of the tick Amblyomma cooperi in the enzootic cycle of Rickettsia rickettsii, the etiologic agent of Brazilian spotted fever (BSF), this study evaluated infection by Rickettsia species in A. cooperi ticks collected from an area in Brazil where BSF is endemic. Among a total of 40 A. cooperi adult ticks collected in an area of BSF endemicity in the state of São Paulo, PCR analysis detected DNA of Rickettsia bellii in 16 ticks (40%), and 3 other ticks (7.5%) were positive for a previously unidentified spotted-fever-group (SFG) rickettsia. Cultivation in Vero cell cultures by the shell vial technique with individual A. cooperi ticks resulted in two isolates of R. bellii and one isolate genotypically characterized as an SFG rickettsia. The two R. bellii isolates were established in Vero cell cultures in the laboratory and were confirmed to be R. bellii by molecular analysis of the gltA and 17-kDa protein-encoding genes and by electron microscopic analysis. The SFG rickettsial isolate could not be stably passaged in cell culture in the laboratory, but molecular analysis of early passages suggested that it was closely related to Rickettsia parkeri, Rickettsia africae, and Rickettsia sibirica. These results do not support the role of A. cooperi in the ecology of R. rickettsii in the area studied, but they add two more species of rickettsiae to the poorly developed list of species occurring in ticks in South America.

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Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs.

Hisashi Inokuma, Masaru Okuda, Koichi Ohno … (2002)

Partial sequences of the 18S rRNA gene (625 bp) from a Hepatozoon detected in two canine hepatozoonosis cases, one clinical and one subclinical, in Japan were analyzed. Both sequences were identical to each other and they were closely related to the Hepatozoon canis strain found in Israel with 99% (617/625) nucleotide identity. Both Hepatozoon americanum and Hepatozoon catasbianae were distantly related to the Japanese Hepatozoon with 94% (586/625) and 91% (566/625) identities, respectively. In a phylogenetic tree, the Japanese Hepatozoon was most closely related to H. canis from Israel but was significantly different than H. americanum and H. catasbianae. These results suggest that the Hepatozoon detected in the Japanese dogs might be a strain variant of H. canis, but is apparently a different species than H. americanum.

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Author and article information

Journal

Journal ID (nlm-ta): J Vet Med Sci

Journal ID (iso-abbrev): J. Vet. Med. Sci

Journal ID (publisher-id): JVMS

Title: The Journal of Veterinary Medical Science

Publisher: The Japanese Society of Veterinary Science

ISSN (Print): 0916-7250

ISSN (Electronic): 1347-7439

Publication date (Electronic): 26 November 2016

Publication date (Print): February 2017

Volume: 79

Issue: 2

Pages: 248-254

Affiliations

[1) ]National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan

[2) ]Department of Disinfection and Vector Control, Nanchang Center for Disease Control and Prevention, Nanchang, Jiangxi 330038, China

[3) ]Nanchang Police Dog Base of the Ministry of Public Security, Nanchang, Jiangxi 330100, China

Author notes

[* ]Correspondence to: Xuan, X., National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. e-mail: gen@ 123456obihiro.ac.jp

Article

Publisher ID: 16-0484

DOI: 10.1292/jvms.16-0484

PMC ID: 5326926

PubMed ID: 27890889

SO-VID: f48c1ce7-bea3-406f-8925-7fbb50037728

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This is an open-access article distributed under the terms of the Creative Commons Attribution Licene (CC-BY-NC-ND 4.0: http://creativecommons.org/licenses/by-nc-nd/4.0/ .

First molecular detection of tick-borne pathogens in dogs from Jiangxi, China

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Abstract

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Ticks and tick-borne pathogens

Most cited references 23

Tick-borne infectious diseases of dogs.

Rickettsia species infecting Amblyomma cooperi ticks from an area in the state of São Paulo, Brazil, where Brazilian spotted fever is endemic.

Analysis of the 18S rRNA gene sequence of a Hepatozoon detected in two Japanese dogs.

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