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      Insertion preference of maize and rice miniature inverted repeat transposable elements as revealed by the analysis of nested elements.

      The Plant cell
      Base Sequence, DNA Transposable Elements, genetics, DNA, Plant, Oryza sativa, Polymerase Chain Reaction, methods, Sequence Analysis, DNA, Zea mays

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          Abstract

          A 128-bp insertion into the maize waxy-B2 allele led to the discovery of Tourist, a family of miniature inverted repeat transposable elements (MITEs). As a special category of nonautonomous elements, MITEs are distinguished by their high copy number, small size, and close association with plant genes. In maize, some Tourist elements (named Tourist-Zm) are present as adjacent or nested insertions. To determine whether the formation of multimers is a common feature of MITEs, we performed a more thorough survey, including an estimation of the proportion of multimers, with 30.2 Mb of publicly available rice genome sequence. Among the 6600 MITEs identified, >10% were present as multimers. The proportion of multimers differs for different MITE families. For some MITE families, a high frequency of self-insertions was found. The fact that all 340 multimers are unique indicates that the multimers are not capable of further amplification.

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          Author and article information

          Journal
          11701888
          139471
          10.1105/tpc.010235

          Chemistry
          Base Sequence,DNA Transposable Elements,genetics,DNA, Plant,Oryza sativa,Polymerase Chain Reaction,methods,Sequence Analysis, DNA,Zea mays

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