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      Identification of a Long-Chain Fatty Acid Elongase from Nannochloropsis sp. Involved in the Biosynthesis of Fatty Acids by Heterologous Expression in Saccharomyces cerevisiae

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          Abstract

          The marine microalga Nannochloropsis sp. contains various elongases and desaturases that are critical for biosynthesis of polyunsaturated fatty acids. A full-length cDNA encoding a long-chain fatty acid elongase, named NsFAE, was cloned from Nannochloropsis sp.. The open reading frame of NsFAE (GenBank accession no. MF680548) consisted of 1068 bp and encoded a predicted protein of 355 amino acids with molecular mass 38.8 kDa. The deduced polypeptide showed 43%–44% identity to fatty acyl elongases from other algae. RT-PCR experiments indicated that the NsFAE gene exhibited the highest expression in Nannochloropsis sp. at 72 h ( i.e., during the third growth stage) and the expression was significantly lower in the other four growth stages. Plasmid p NsFAE-CRISPR and a recombinant DNA fragment (ADH 1p- NsFAE-CYCt) were transformed into Saccharomyces cerevisiae strain BY4742 using the CRISPR-Cas system. Yeast transformants containing NsFAE produced three fatty acids not normally present in wild-type BY4742–linoleic acid, linolenic acid and eicosadienoic acid-indicating that NsFAE encodes a functional elongase enzyme.

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          Author and article information

          Journal
          JOUC
          Journal of Ocean University of China
          Science Press and Springer (China )
          1672-5182
          24 August 2019
          01 October 2019
          : 18
          : 5
          : 1199-1206
          Affiliations
          1College of Food, Shihezi University, Shihezi 832000, China
          2The First Institute of Oceanography, State Oceanic Administration of China, Qingdao 266061, China
          Author notes
          *Corresponding author: CHEN Guogang
          Article
          s11802-019-3946-y
          10.1007/s11802-019-3946-y
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2019.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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          Self URI (journal-page): https://www.springer.com/journal/11802

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