The Protective Effect of Echinochrome A on Extracellular Matrix of Vocal Folds in Ovariectomized Rats

There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

Abstract

Here, we investigated the effects of sex hormones on extracellular matrix (ECM)-related gene expression in the vocal fold lamina propria of ovariectomized (after ovary removal) rats and verified whether echinochrome A (ECH) exerts any therapeutic effects on ECM reconstitution after estrogen deficiency in ovariectomized rats. Sprague–Dawley female rats (9 weeks old) were acclimatized for a week and randomly divided into three groups ( n = 15 each group) as follows: group I (sham-operated rats, SHAM), group II (ovariectomized rats, OVX), group III (ovariectomized rats treated with ECH, OVX + ECH). Rats from the OVX + ECH group were intraperitoneally injected with ECH at 10 mg/kg thrice a week after surgery for 6 weeks. And rats were sacrificed 6 weeks after ovariectomy. Estradiol levels decreased in OVX group compared with the SHAM group. ECH treatment had no effect on the levels of estradiol and expression of estrogen receptor β (ERβ). The evaluation of ECM components showed no significant changes in elastin and hyaluronic acid levels between the different groups. Collagen I and III levels were lower in OVX group than in SHAM group but increased in OVX + ECH group. The mRNA levels of matrix metalloproteinase (MMP)-1, -2, -8, and -9 were significantly higher in the OVX group than in the SHAM group, but decreased in the OVX + ECH group. Thus, changes were observed in ECM-related genes in the OVX group upon estradiol deficiency that were ameliorated by ECH administration. Thus, the vocal fold is an estradiol-sensitive target organ and ECH may have protective effects on the ECM of vocal folds in ovariectomized rats.

Related collections

Most cited references 37

Record: found
Abstract: found
Article: not found

Alternative and complementary therapies for the menopause.

Francesca Borrelli, Edzard Ernst (2010)

The use of complementary and alternative medicine (CAM) among menopausal women has increased in the last years. This review examines the evidence from systematic reviews, RCTs and epidemiological studies of CAM in the treatment of menopausal symptoms. Some evidence exists in favour of phytosterols and phytostanols for diminishing LDL and total cholesterol in postmenopausal women. Similarly, regular fiber intake is effective in reducing serum total cholesterol in hypercholesterolemic postmenopausal women. Clinical evidence also exists on the effectiveness of vitamin K, a combination of calcium and vitamin D or a combination of walking with other weight-bearing exercise in reducing bone mineral density loss and the incidence of fractures in postmenopausal women. Black cohosh appears to be effective therapy for relieving menopausal symptoms, primarily hot flashes, in early menopause. Phytoestrogen extracts, including isoflavones and lignans, appear to have only minimal effect on hot flashes but have other positive health effects, e.g. on plasma lipid levels and bone loss. For other commonly used CAMs, e.g. probiotics, prebiotics, acupuncture, homeopathy and DHEA-S, randomized, placebo-controlled trials are scarce and the evidence is unconvincing. More and better RCTs testing the effectiveness of these treatments are needed. Copyright 2010 Elsevier Ireland Ltd. All rights reserved.

0 comments Cited 48 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Airway glutathione homeostasis is altered in children with severe asthma: evidence for oxidant stress.

Anne Fitzpatrick, W. Gerald Teague, Fernando Holguin … (2009)

Severe asthma is characterized by persistent airway inflammation and increased formation of reactive oxygen species. Glutathione (GSH) is an important antioxidant in the epithelial lining fluid (ELF). We hypothesized that airway GSH homeostasis was altered in children with severe asthma and was characterized by decreased GSH and increased glutathione disulfide (GSSG) concentrations. Bronchoalveolar lavage was obtained from 65 children with severe asthma, including 35 children with baseline airway obstruction evidenced by FEV(1) <80%. Control data were obtained from 6 children with psychogenic (habit) cough or vocal cord dysfunction undergoing diagnostic bronchoscopy and 35 healthy adult controls. GSH, GSSG, and other determinants of airway oxidative stress including glutathione S-transferase (GST), glutathione reductase (GR), glutathione peroxidase (GPx), malondialdehyde, 8-isoprostane, and H(2)O(2) were measured in the ELF. The ELF redox potential was calculated from GSH and GSSG by using the Nernst equation. Compared with controls, subjects with severe asthma had lower airway GSH with increased GSSG despite no differences in GST, GR, and GPx activities between groups. This was accompanied by increased malondialdehyde, 8-isoprostane, and H(2)O(2) concentrations in the ELF. GSH oxidation was most apparent in subjects with severe asthma with airway obstruction and was supported by an upward shift in the ELF GSH redox potential. Children with severe asthma have increased biomarkers of oxidant stress in the ELF that are associated with increased formation of GSSG and a shift in the GSH redox potential toward the more oxidized state.

0 comments Cited 38 times – based on 0 reviews      Review now

Bookmark

Record: found
Abstract: found
Article: not found

Current understanding and review of the literature: vocal fold scarring.

Christopher Hansen, Susan Thibeault (2006)

Vocal fold scarring is the greatest cause of poor voice after vocal fold injury. Scarring causes a disruption of the viscoelastic layered structure of the lamina propria, an increase in stiffness of the vibratory structure, and glottic incompetence. Current treatments for this complex condition are inconsistent and often produce suboptimal results. Research investigating this condition has dramatically increased over the last several years. The literature has been directed toward understanding vocal fold scarring at the biological level and translating this to the clinical forum. We present an up-to-date, thorough, and scholarly review of the literature in vocal fold scarring since 1996.

0 comments Cited 24 times – based on 0 reviews      Review now

Bookmark

All references

Author and article information

Journal

Journal ID (nlm-ta): Mar Drugs

Journal ID (iso-abbrev): Mar Drugs

Journal ID (publisher-id): marinedrugs

Title: Marine Drugs

Publisher: MDPI

ISSN (Electronic): 1660-3397

Publication date (Electronic): 24 January 2020

Publication date Collection: February 2020

Volume: 18

Issue: 2

Electronic Location Identifier: 77

Affiliations

[1 ]Pusan National University School of Medicine and Medical Research Institute, Pusan National University, Yangsan 50612, Korea; ny5thav@ 123456hanmail.net

[2 ]Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea; kmky82@ 123456naver.com

[3 ]Department of Otorhinolaryngology-Head and Neck Surgery and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea; cha-nwi@ 123456daum.net

[4 ]Department of Otolaryngology-Head and Neck Surgery, Samsung Changwon Hospital, Sungkyunkwan University School of Medicine, Changwon 51353, Korea; uuhent@ 123456gmail.com

[5 ]Department of Life Science in Dentistry, School of Dentistry, Pusan National University, Yangsan 50612, Korea; hskimcell@ 123456pusan.ac.kr

[6 ]Institute for Translational Dental Science, Pusan National University, Yangsan 50612, Korea

[7 ]Department of Nuclear Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan 49241, Korea; buisket@ 123456naver.com

[8 ]National Research Laboratory for Mitochondrial Signaling, Department of Physiology, College of Medicine, Cardiovascular and Metabolic Disease Center (CMDC), Inje University, Busan 47391, Korea; estrus74@ 123456gmail.com (H.K.K.); phyhanj@ 123456inje.ac.kr (J.H.)

[9 ]G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch of the Russian Academy of Science, 690022 Vladivostok, Russia; mischenkonp@ 123456mail.ru (N.P.M.); vasilieva_el_an@ 123456mail.ru (E.A.V.); fedoreev-s@ 123456mail.ru (S.A.F.); stonik@ 123456piboc.dvo.ru (V.A.S.)

Author notes

[* ]Correspondence: voiceleebj@ 123456gmail.com ; Tel.: +82-51-240-7528; Fax: +82-51-240-2162

Author information

Ji Min Kim https://orcid.org/0000-0002-3608-765X

Jeong Hun Kim https://orcid.org/0000-0002-1534-0062

Gi Cheol Park https://orcid.org/0000-0001-7346-8550

Natalia P. Mishchenko https://orcid.org/0000-0001-7616-574X

Elena A. Vasileva https://orcid.org/0000-0001-7526-026X

Byung-Joo Lee https://orcid.org/0000-0001-7091-6688

Article

Publisher ID: marinedrugs-18-00077

DOI: 10.3390/md18020077

PMC ID: 7073970

PubMed ID: 31991543

SO-VID: f6313306-b31a-433d-9947-24f7084ee475

License:

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

The Protective Effect of Echinochrome A on Extracellular Matrix of Vocal Folds in Ovariectomized Rats

Read this article at

Abstract

Related collections

Herbal Medicines Journal (Herb Med J)

Most cited references 37

Alternative and complementary therapies for the menopause.

Airway glutathione homeostasis is altered in children with severe asthma: evidence for oxidant stress.

Current understanding and review of the literature: vocal fold scarring.

Author and article information

Journal

Affiliations

Author notes

Author information

Article

History

Categories

Comments

Comment on this article

Similar content 657

Cited by 2

Most referenced authors 437