For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
27
views
22
references
 Top references
cited by
11
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      117
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Multiplex PCR Assay for Unequivocal Differentiation of Actinobacillus pleuropneumoniae Serovars 1 to 3, 5 to 8, 10, and 12

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          An improved multiplex PCR, using redesigned primers targeting the serovar 3 capsule locus, which differentiates serovars 3, 6, and 8 Actinobacillus pleuropneumoniae isolates, is described. The new primers eliminate an aberrant serovar 3-indicative amplicon found in some serovar 6 clinical isolates. Furthermore, we have developed a new multiplex PCR for the detection of serovars 1 to 3, 5 to 8, 10, and 12 along with apxIV, thus extending the utility of this diagnostic PCR to cover a broader range of isolates.

          Related collections

          Most cited references22

          • Record: found
          • Abstract: found
          • Article: not found

          A large genome center's improvements to the Illumina sequencing system.

          Michael Quail, Iwanka Kozarewa, Frances Smith … (2008)
          The Wellcome Trust Sanger Institute is one of the world's largest genome centers, and a substantial amount of our sequencing is performed with 'next-generation' massively parallel sequencing technologies: in June 2008 the quantity of purity-filtered sequence data generated by our Genome Analyzer (Illumina) platforms reached 1 terabase, and our average weekly Illumina production output is currently 64 gigabases. Here we describe a set of improvements we have made to the standard Illumina protocols to make the library preparation more reliable in a high-throughput environment, to reduce bias, tighten insert size distribution and reliably obtain high yields of data.
          Article 0 comments Cited 300 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Actinobacillus pleuropneumoniae: pathobiology and pathogenesis of infection.

            Janine Bossé, Paul R. Langford, J. Kroll … (2002)
            Actinobacillus pleuropneumoniae causes porcine pleuropneumonia, a highly contagious disease for which there is no effective vaccine. This review considers how adhesins, iron-acquisition factors, capsule and lipopolysaccharide, RTX cytotoxins and other potential future vaccine components contribute to colonisation, to avoidance of host clearance mechanisms and to damage of host tissues.
            Article 0 comments Cited 101 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Improved Protocols for Illumina Sequencing.

              Iraad F. Bronner, Michael Quail, Daniel J. Turner … (2014)
              In this unit, we describe a set of improvements that have been made to the standard Illumina protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data.
              Article 0 comments Cited 40 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Contributors
                E. Munson: Role: Editor
                Journal
                Journal ID (nlm-ta): J Clin Microbiol
                Journal ID (iso-abbrev): J. Clin. Microbiol
                Journal ID (hwp): jcm
                Journal ID (pmc): jcm
                Journal ID (publisher-id): JCM
                Title: Journal of Clinical Microbiology
                Publisher: American Society for Microbiology (1752 N St., N.W., Washington, DC )
                ISSN (Print): 0095-1137
                ISSN (Electronic): 1098-660X
                Publication date (Print): July 2014
                Publication date PMC-release: July 2014
                Volume: 52
                Issue: 7
                Pages: 2380-2385
                Affiliations
                [a ]Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom
                [b ]Norwegian Veterinary Institute, Oslo, Norway
                [c ]Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom
                [d ]The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Cambridge, United Kingdom
                [e ]Animal Health Veterinary Laboratories Agency (AHVLA), Rougham Hill, Suffolk, United Kingdom
                [f ]Department of Pathogen Molecular Biology, London School of Hygiene and Tropical Medicine, London, United Kingdom
                [g ]Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, Hertfordshire, United Kingdom
                Author notes
                Address correspondence to Paul R. Langford, p.langford@ 123456imperial.ac.uk .

                J.T.B and Y.L. contributed equally to this work.

                [*]

                Present address: Roy R. Chaudhuri, Department of Molecular Biology and Biotechnology, University of Sheffield, Sheffield, United Kingdom; Matthew T. Holden, School of Medicine, University of St. Andrews, Fife, United Kingdom.

                Article
                Publisher ID: 00685-14
                DOI: 10.1128/JCM.00685-14
                PMC ID: 4097740
                PubMed ID: 24759717
                SO-VID: f632927c-73b1-40ca-9f35-910ec7fa81e2
                Copyright © Copyright © 2014 Bossé et al.
                License:

                This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 Unported license.

                History
                Date received : 19 March 2014
                Date revision requested : 31 March 2014
                Date accepted : 15 April 2014
                Categories
                Subject: Clinical Veterinary Microbiology

                ScienceOpen disciplines: Microbiology & Virology
                Data availability:
                ScienceOpen disciplines: Microbiology & Virology

                Comments

                Comment on this article

                scite_

                Similar content117

                See all similar

                Cited by11

                See all cited by

                Most referenced authors610

                See all reference authors