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      Perinatal Hypothyroidism Decreases Hippocampal 3H-Vinylidene Kainic Acid Binding in Rats

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          Abstract

          The effect of perinatal hypothyroidism on the number and distribution of hippocampal kainic acid binding sites was examined in rats. Timed pregnant Sprague-Dawley rat dams were given water containing either 0.02% propylthiouracil (PTU) or untreated water from gestational day 18 until their litters were weaned at postnatal day 31. The offspring were sacrificed at 31 or 120 days of age, blood samples collected and their brains frozen. In the 31-day-old rats, serum thyroxine, serum triiodothyronine, total body weight and whole brain weight all indicated that the PTU-treated rats were hypothyroid. Hippocampal kainic acid binding was analyzed in sections of dorsal and ventral hippocampal formation by in vitro <sup>3</sup>H-vinylidene kainic acid (VKA) autoradiography. Compared to the untreated controls, specific <sup>3</sup>H-VKA binding was reduced by 43% in the ventral hippocampal formation stratum lucidum of 31-day-old FΓU-treated rats. Specific <sup>3</sup>H-VKA binding was not different in the dorsal hippocampal formation. Saturation of <sup>3</sup>H-VKA binding studies indicated that the decrease – induced by PTU treatment – in ventral hippocampal <sup>3</sup>H-VKA binding was due to a reduction in the total number of <sup>3</sup>H-VKA binding sites. At 120 days of age, 3 months after the cessation of the PTU treatment, serum thyroid hormone levels were not different than those of controls, indicating a recovery of thyroid hormone function after the perinatal PTU treatment. However, specific <sup>3</sup>H-VKA binding remained significantly reduced in the ventral hippocampal formation of 120-day-old rats. This observation indicated that the decrease in <sup>3</sup>H-VKA binding at 31 days of age was not simply due to a delay in the development of this system and that perinatal hypothyroidism can cause long-lasting alterations in the total number of hippocampal kainic acid binding sites.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1990
          1990
          03 April 2008
          : 51
          : 1
          : 38-44
          Affiliations
          Department of Pharmacology, University of New Mexico School of Medicine, Albuquerque, N. Mex., USA
          Article
          125313 Neuroendocrinology 1990;51:38–44
          10.1159/000125313
          2154712
          f641395d-3a4b-475d-b1a2-f9c6dd05d451
          © 1990 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          History
          : 20 March 1989
          : 19 May 1989
          Page count
          Pages: 7
          Categories
          Original Paper

          Endocrinology & Diabetes,Neurology,Nutrition & Dietetics,Sexual medicine,Internal medicine,Pharmacology & Pharmaceutical medicine
          Hypothyroidism,Kainic acid receptors,Hippocampal formation

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