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      A mesoscale connectome of the mouse brain

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          Abstract

          Comprehensive knowledge of the brain’s wiring diagram is fundamental for understanding how the nervous system processes information at both local and global scales. However, with the singular exception of the C. elegans microscale connectome, there are no complete connectivity data sets in other species. Here we report a brain-wide, cellular-level, mesoscale connectome for the mouse. The Allen Mouse Brain Connectivity Atlas uses enhanced green fluorescent protein (EGFP)-expressing adeno-associated viral vectors to trace axonal projections from defined regions and cell types, and high-throughput serial two-photon tomography to image the EGFP-labelled axons throughout the brain. This systematic and standardized approach allows spatial registration of individual experiments into a common three dimensional (3D) reference space, resulting in a whole-brain connectivity matrix. A computational model yields insights into connectional strength distribution, symmetry and other network properties. Virtual tractography illustrates 3D topography among interconnected regions. Cortico-thalamic pathway analysis demonstrates segregation and integration of parallel pathways. The Allen Mouse Brain Connectivity Atlas is a freely available, foundational resource for structural and functional investigations into the neural circuits that support behavioural and cognitive processes in health and disease.

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          Author and article information

          Journal
          0410462
          6011
          Nature
          Nature
          Nature
          0028-0836
          1476-4687
          3 November 2016
          02 April 2014
          10 April 2014
          09 November 2016
          : 508
          : 7495
          : 207-214
          Affiliations
          [1 ]Allen Institute for Brain Science, Seattle, Washington 98103, USA
          [2 ]Laboratory of Systems Neuroscience, National Institute of Mental Health, Bethesda, Maryland 20892, USA
          Author notes
          Correspondence and requests for materials should be addressed to H.Z. ( hongkuiz@ 123456alleninstitute.org )
          [*]

          These authors contributed equally to this work.

          Article
          PMC5102064 PMC5102064 5102064 nihpa716676
          10.1038/nature13186
          5102064
          24695228
          f6584c6d-8cbd-45cf-be94-560bba56eb8d

          Reprints and permissions information is available at www.nature.com/reprints.

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