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      Simultaneous Quantification of κ-Carrageenan Oligosaccharides of DP 3, 5 and 7 by LC-MS/MS: Application to an in vitro Absorption Study

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          Carrageenans are widely utilized in many commercial applications such as the food and pharmaceutical industry, due to their excellent functional properties. In this study, a sensitive LC-MS/MS method was developed to determine κ-3, κ-5, and κ-7 carrageenan oligosaccharides simultaneously. Optimum MRM transitions for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were (645.079→565.111, [M-H] ), (515.137→474.946, [M-2H] 2−), and (471.484→445, [M-3H] 3−), respectively. Chromatographic separation was performed on an Amide column coupled with a guard column operated at 60°C under stepwise gradient elution. The linearity of the LC-MS/MS method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides, evaluated over the concentration range of 0.10–20.0 μmol L −1, was excellent. The precisions of the method for κ-3, κ-5, and κ-7 carrageenan oligosaccharides were from 0.91% to 9.66%, and the inter-day precisions were from 0.92% to 10.5%. Validation of the LC-MS/MS method indicated that the method was precise and in line with the CFDA guidance. This method has been successfully applied to an in vitro absorption study.

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          Author and article information

          Journal of Ocean University of China
          Science Press and Springer (China )
          02 September 2020
          01 October 2020
          : 19
          : 5
          : 1177-1182
          1School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China
          2Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266003, China
          3Key Laboratory of Glycoscience & Glycotechnology of Shandong Province, Qingdao 266003, China
          4Key Laboratory of Marine Drugs, Ministry of Education of China, Qingdao 266003, China
          Author notes
          *Corresponding authors: LV Zhihua, E-mail: lvzhihua@ ; YU Mingming, E-mail: yumingming@
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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