Validation of disinfection effect of Vesphene llse and VirkonS disinfectant on Mycobacterium tuberculosis

Abstract

Objective To verify the bactericidal ability of Vesphene Ⅱ se and VirkonS disinfectant against Mycobacterium tuberculosis, and we seek the best disinfection effect of tuberculosis laboratory.

Methods Suspension quantitative germicidal test was used to determine the germicidal efficacy of 5, 10 and 20 minutes of 1:85, 1:128, 1:256 Vesphene Ⅱ se disinfectant and 1:66, 1:100, 1:133, 1:200, 1:400 VirkonS disinfectant against Mycobacterium tuberculosis. Each test was repeated 3 times.

Results The 1:85 concentration Vesphene Ⅱ se disinfectant can kill Mycobacterium tuberculosis within 5 minutes, but can not completely kill Mycobacterium tuberculosis within 10, 20 minutes; 1:128 concentration disinfection can not completely kill Mycobacterium tuberculosis within 5 minutes, disinfection can completely kill Mycobacterium tuberculosis within 10 minutes and 20 minutes; 1:256 concentration Vesphene Ⅱ se disinfectant can not completely kill Mycobacterium tuberculosis within 5, 10, and 20 minutes; while 1:66, 1:100, 1:133, 1:200 and 1:400 concentration VirkonS disinfectant can not completely kill Mycobacterium tuberculosis within 5, 10, and 20 minutes.

Conclusion The Vesphene IIse disinfectant with a concentration of 1:128 can effectively kill Mycobacterium tuberculosis and can be used as a disinfectant in the tuberculosis laboratory for 10 minutes; the VirkonS disinfectant, though a broad-spectrum disinfectant, is not sensitive to Mycobacterium tuberculosis and has poor killing efficiency, so it can not be used as a disinfectant in the tuberculosis laboratory.

Abstract

摘要： 目的 验证碱性苯酚和卫可消毒剂对结核分枝杆菌的杀菌能力, 以寻求结核菌实验室最佳消毒效果。 方法 悬液定量杀灭试验测定5、10、20min, 浓度为1：85、1：128、1：256的碱性苯酚消毒剂和浓度为1:66、1:100、 1：133、1：200、1：400的卫可消毒剂对结核分枝杆菌的杀菌能力。各试验重复3次。 结果 1:85碱性苯酚消毒剂消毒 5min可以完全杀灭结核分枝杆菌, 但是消毒10、20min不能完全杀灭结核分枝杆菌；1：128消毒5 min内不能完全杀灭 结核分枝杆菌, 消毒10 min和20 min可以完全杀灭结核分枝杆菌；1:256碱性苯酚消毒剂消毒5、10、20min均不能完全 杀灭结核分枝杆菌；而1:66、1:100、1:133、1:200和1:400的卫可消毒剂消毒5、10、20min均不能完全杀灭结核分枝杆 菌。 结论 浓度为1：128的碱性苯酚消毒剂作用10 min可以有效杀灭结核分枝杆菌, 可以作为结核实验室消毒剂；卫 可消毒剂虽为广谱消毒剂但对结核分枝杆菌不敏感, 杀灭效率差, 不可作为结核实验室消毒剂。