The concentrations of 19 amino acids and related compounds were simultaneously determined in the aqueous humour (C<sub>aq</sub>) and plasma (C<sub>pl</sub>) of 40 patients with various ocular disorders, by means of ion-exchange chromatography. The purpose of the study was to elucidate the transport across the blood-aqueous barrier in man by studying the relationship between C<sub>aq</sub> and C<sub>pl</sub> for each substance. Graphs of C<sub>aq</sub> versus C<sub>pl</sub> revealed for most substances a positive correlation, supported by a statistical regression analysis. It may be concluded by the use of a simple kinetic model that the transport of threonine, glutamine (containing serine and asparagine), α-aminobutyric acid, valine, methionine, isoleucine, leucine, tyrosine, phenylalanine, arginine, proline, alanine, lysine and histidine must in part take place by means of secretion, whereas for taurine, urea, glutamic acid, glycine and cystine no such evidence for secretion has been found, but neither has it been excluded.