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      Effect of sodium butyrate and zinc sulphate supplementation on recombinant human IFN-β production by mammalian cell culture

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          Abstract

          Recombinant human interferon-β1 (rhIFN-β1) glycoprotein is used as a therapeutic agent for a variety of diseases, such as multiple sclerosis and hepatitis. In the present work, different strategies were applied to produce rhIFN-β1a in mammalian cell cultures. Transfected population of CHO-K1, CHO dhfr-, BHK and HEK cells were compared for their ability to produce rhIFN-β1a, and clones of the most promising cell line (CHO-K1) were isolated by the limit dilution method. Likewise, different culture conditions were assayed by changing the amounts of fetal calf serum, sodium butyrate and/or ZnSO4, to improve cell productivity. The presence of each additive increased the rhIFN-β1a yield ranging from 2 to 8 times, depending on the tested cell clone, but when these components were simultaneously added to the medium, the rhIFN-β1a concentration in the supernatants was even greater.

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          Most cited references30

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          Cytokine therapeutics: lessons from interferon alpha.

          Cytokines are soluble proteins that allow for communication between cells and the external environment. Interferon (IFN) alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. This review focuses on the biological and clinical activities of the cytokine. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. The principles learned from in vivo studies will be discussed, particularly hairy cell leukemia, chronic myelogenous leukemia, certain angiogenic diseases, and hepatitis. After the surprising discovery of activity in a rare B-cell neoplasm, IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation. Regulatory agencies throughout the world have approved IFN-alpha for treatment of 13 malignant and viral disorders. The principles established with this cytokine serve as a paradigm for future development of natural proteins for human disease.
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            Culture of Animal Cells-A Manual of Basic Technique

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              Human GM-CSF: molecular cloning of the complementary DNA and purification of the natural and recombinant proteins.

              Clones of complementary DNA encoding the human lymphokine known as granulocyte-macrophage colony-stimulating factor (GM-CSF) were isolated by means of a mammalian cell (monkey COS cell) expression screening system. One of these clones was used to produce recombinant GM-CSF in mammalian cells. The recombinant hematopoietin was similar to the natural product that was purified to apparent homogeneity from medium conditioned by a human T-cell line. The human T-cell GM-CSF was found to be 60 percent homologous with the GM-CSF recently cloned from murine lung messenger RNA.
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                laar
                Latin American applied research
                Lat. Am. appl. res.
                Universidad Nacional del Sur y Consejo Nacional de Investigaciones Científicas y Técnicas (Bahía Blanca )
                1851-8796
                October 2006
                : 36
                : 4
                : 321-327
                Affiliations
                [1 ] Universidad Nacional del Litoral Argentina
                Article
                S0327-07932006000400018
                f6fba801-ef38-4b6e-82e5-a0b76e967d9c

                http://creativecommons.org/licenses/by/4.0/

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                Product

                SciELO Argentina

                Self URI (journal page): http://www.scielo.org.ar/scielo.php?script=sci_serial&pid=0327-0793&lng=en
                Categories
                ENGINEERING, CHEMICAL

                General engineering
                rhIFN-β1a Production,CHO Cells,Zinc Sulphate,Sodium Butyrate
                General engineering
                rhIFN-β1a Production, CHO Cells, Zinc Sulphate, Sodium Butyrate

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