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      Seroprevalencia de anticuerpos anti-Brucella en disponentes de sangre con fines terapéuticos en tres bancos de sangre del Instituto Mexicano del Seguro Social Translated title: Anti-Brucella Antibody Seroprevalence in Blood Donors for Therapeutic Ends at Three Blood Banks of the Mexican Institute of Social Security

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          Abstract

          Introducción: la brucelosis es una zoonosis, que causa grandes pérdidas económicas en las zonas conurbanas de la Ciudad de México y es un problema importante de salud pública en los habitantes circunvecinos al Distrito Federal. El objetivo fue detectar anticuerpos anti-Brucella y según los resultados que proporcionó esta investigación, se propone como prueba de laboratorio de escrutinio en los donadores de sangre. Material y métodos: se analizaron 500 sueros sanguíneos de disponentes efectivos seleccionados y cuya muestra fue representativa de acuerdo al análisis estadístico elaborado. Las pruebas de laboratorio incluyeron Rosa de Bengala, Aglutinación Estándar en Microplaca y 2 Mercaptoetanol. Resultados: de los 500 sueros analizados 18 mostraron seropositividad con una tasa de seroprevalencia de 3.6%, predominando el sexo masculino (83.4%), por grupo de actividad las secundarias (72.2%), por grado de estudios académicos los de secundaria fueron los de mayor positividad (55.6%). Conclusión: la brucelosis posee características epidemiológicas peculiares en los bancos de sangre participantes en esta investigación, por lo que es importante incluir pruebas de escrutinio en búsqueda de anticuerpos anti-Brucella en los disponentes de sangre efectivos.

          Translated abstract

          Introduction: to determine seroprevalence for Brucella sp. in blood donors, a serologic study was carried out at three blood banks of the Instituto Mexicano del Seguro Social (IMSS). Methods: 500 blood samples were taken from selected blood donors. Laboratory tests were used, such as Bengal rose (BR), Standard agglutination in microplate (SAM) and in presence of 2-Mercaptoethanol agglutination in microplate (2ME), which were applied to 500 blood sera from selected effective blood donors. The sample was representative according to the statistical analysis developed. Results: 18 of 500 analyzed sera were positive, with seroprevalence of 3.6%, male sex (83.4%), predominating, as secondary activity group (72.2%). According to academic archivement, blood donors with secondary school had highest seropositivity (55.6%). Conclusion: In this study, we conclude that brucellosis has peculiar epidemiologic characteristics in blood banks that participated in this research; therefore it is highly recommended to perform screening tests such as BR, SAM, and 2ME to identified anti-Brucella antibodies in the sera of effective blood donors

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          Most cited references 43

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          An overview of human brucellosis.

           Susan Young (1995)
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            Characteristics of a Brucella species from a bottlenose dolphin (Tursiops truncatus).

            A culture isolated from an aborted fetus of a bottlenose dolphin (Tursiops truncatus) was characterized. The isolate was a gram-negative coccobacillus, and the colonial morphology was typical of a smooth Brucella. The isolate was positive for catalase, oxidase, nitrate reduction, and urease. Hydrogen sulfide was not produced. It grew in air at 37 C but required 72 hours for good growth. There was growth on media containing basic fuchsin, thionin, thionin blue, penicillin, and erythritol. The M antigen was dominant, and the isolate was lysed by 4 of 10 brucellaphages tested. The oxidative metabolic profile of the isolate was similar to that for B. abortus but differed in utilization of L-asparagine, L-glutamic acid, and DL-citrulline. Whole-cell lysates were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The protein profiles were markedly different from the protein profiles of reference strains of Brucella species. Biochemical and oxidative metabolism profiles indicated that the isolate belongs in the genus Brucella but did not match the profiles of any established species or biovars. This isolate may be an atypical strain of a recognized Brucella species or a new biovar or species of Brucella.
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              Detection of Brucellae in blood cultures.

               P Yagupsky (1999)
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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                gmm
                Gaceta médica de México
                Gac. Méd. Méx
                Academia Nacional de Medicina de México, A.C. (México )
                0016-3813
                August 2004
                : 140
                : 4
                : 391-398
                Affiliations
                [1 ] Instituto Mexicano del Seguro Social Mexico
                [2 ] Instituto Politécnico Nacional Mexico
                Article
                S0016-38132004000400004

                http://creativecommons.org/licenses/by/4.0/

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                Categories
                Medicine, General & Internal

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