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      Analysis of Molecular Masses and Oligomeric States of Protein Complexes by Blue Native Electrophoresis and Isolation of Membrane Protein Complexes by Two-Dimensional Native Electrophoresis

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      Analytical Biochemistry
      Elsevier BV

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          Abstract

          Blue native Electrophoresis is a "charge shift" method developed for isolation of native membrane protein complexes from biological membranes that also separates both acidic and basic water-soluble proteins at a fixed pH of 7.5. In combination with a second dimension sodium dodecylsulfate electrophoresis it provides an analytical method for the determination of molecular mass and oligomeric state of nondissociated complexes, of subunit composition, and of degree of purity and for the detection of subcomplexes. The method was applied to analysis of cytochrome bc/bf complexes. By combination of a novel colorless native polyacrylamide gel electrophoresis (CN-PAGE) with blue native BN-PAGE, a two-dimensional native technique was developed that is suitable for preparation of highly pure membrane protein complexes.

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          Author and article information

          Journal
          Analytical Biochemistry
          Analytical Biochemistry
          Elsevier BV
          00032697
          March 1994
          March 1994
          : 217
          : 2
          : 220-230
          Article
          10.1006/abio.1994.1112
          8203750
          f715a395-5a7a-41a8-8bf8-034f1bb90ff2
          © 1994

          https://www.elsevier.com/tdm/userlicense/1.0/

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