Implications for human odor sensing revealed from the statistics of odorant-receptor interactions

Abstract

Binding of odorants to olfactory receptors (ORs) elicits downstream chemical and neural signals, which are further processed to odor perception in the brain. Recently, Mainland and colleagues have measured more than 500 pairs of odorant-OR interaction by a high-throughput screening assay method, opening a new avenue to understanding the principles of human odor coding. Here, using a recently developed minimal model for OR activation kinetics, we characterize the statistics of OR activation by odorants in terms of three empirical parameters: the half-maximum effective concentration EC ₅₀, the efficacy, and the basal activity. While the data size of odorants is still limited, the statistics offer meaningful information on the breadth and optimality of the tuning of human ORs to odorants, and allow us to relate the three parameters with the microscopic rate constants and binding affinities that define the OR activation kinetics. Despite the stochastic nature of the response expected at individual OR-odorant level, we assess that the confluence of signals in a neuron released from the multitude of ORs is effectively free of noise and deterministic with respect to changes in odorant concentration. Thus, setting a threshold to the fraction of activated OR copy number for neural spiking binarizes the electrophysiological signal of olfactory sensory neuron, thereby making an information theoretic approach a viable tool in studying the principles of odor perception.

Author summary

Despite the decades of research, quantitative details of human olfaction have remained largely unexplored. However, a high-throughput measurement has recently been carried out to produce dose-response data between a set of odorants and a repertoire of human olfactory receptors. We characterized each pair of odorant-receptor interaction in terms of EC50, efficacy, and basal level, a strategy often adopted in biochemical, pharmacological sciences to describe the response of receptors to cognate ligands. The distributions of EC50 values and efficacies acquired from the analysis provide glimpses into how human olfactory receptors are tuned to odorants. Specifically, the response of human ORs is optimized around ∼ 100 μM of odorant. Next, the efficacies of OR responses to odorants are bi-exponentially distributed, which indicates that the strength of odorant-OR interaction is classified into strong and weak subgroups. By showing that the stochastic response of individual receptor to odorant can effectively be binarized at cellular level through olfactory processes, we also provide a theoretical basis for an information theoretical approach in studying the principles of odor perception.