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      The STE20/germinal center kinase POD6 interacts with the NDR kinase COT1 and is involved in polar tip extension in Neurospora crassa.

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          Abstract

          Members of the Ste20 and NDR protein kinase families are important for normal cell differentiation and morphogenesis in various organisms. We characterized POD6 (NCU02537.2), a novel member of the GCK family of Ste20 kinases that is essential for hyphal tip extension and coordinated branch formation in the filamentous fungus Neurospora crassa. pod-6 and the NDR kinase mutant cot-1 exhibit indistinguishable growth defects, characterized by cessation of cell elongation, hyperbranching, and altered cell-wall composition. We suggest that POD6 and COT1 act in the same genetic pathway, based on the fact that both pod-6 and cot-1 can be suppressed by 1) environmental stresses, 2) altering protein kinase A activity, and 3) common extragenic suppressors (ropy, as well as gul-1, which is characterized here as the ortholog of the budding and fission yeasts SSD1 and Sts5, respectively). Unlinked noncomplementation of cot-1/pod-6 alleles indicates a potential physical interaction between the two kinases, which is further supported by coimmunoprecipitation analyses, partial colocalization of both proteins in wild-type cells, and their common mislocalization in dynein/kinesin mutants. We conclude that POD6 acts together with COT1 and is essential for polar cell extension in a kinesin/dynein-dependent manner in N. crassa.

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          Author and article information

          Journal
          Mol Biol Cell
          Molecular biology of the cell
          American Society for Cell Biology (ASCB)
          1059-1524
          1059-1524
          Sep 2006
          : 17
          : 9
          Affiliations
          [1 ] Deutsche Forschungsgemeinschaft Research Center of Molecular Physiology of the Brain (CMPB), Abteilung Molekulare Mikrobiologie, Universität Göttingen, D-37077 Göttingen, Germany. sseiler@gwdg.de
          Article
          E06-01-0072
          10.1091/mbc.e06-01-0072
          1593175
          16822837
          f722804e-b03c-473f-bf13-efefff73640c
          History

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