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      The green fluorescent protein.

      Annual review of biochemistry

      chemistry, Luminescent Proteins, Indicators and Reagents, Green Fluorescent Proteins, Genes, Reporter, Biological Markers

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          Abstract

          In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.

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          Author and article information

          Journal
          10.1146/annurev.biochem.67.1.509
          9759496

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