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      The green fluorescent protein.

      1
      Annual review of biochemistry
      Annual Reviews

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          Abstract

          In just three years, the green fluorescent protein (GFP) from the jellyfish Aequorea victoria has vaulted from obscurity to become one of the most widely studied and exploited proteins in biochemistry and cell biology. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. High-resolution crystal structures of GFP offer unprecedented opportunities to understand and manipulate the relation between protein structure and spectroscopic function. GFP has become well established as a marker of gene expression and protein targeting in intact cells and organisms. Mutagenesis and engineering of GFP into chimeric proteins are opening new vistas in physiological indicators, biosensors, and photochemical memories.

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          Author and article information

          Journal
          Annu Rev Biochem
          Annual review of biochemistry
          Annual Reviews
          0066-4154
          0066-4154
          1998
          : 67
          Affiliations
          [1 ] Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093-0647, USA.
          Article
          10.1146/annurev.biochem.67.1.509
          9759496
          f753efb1-24d5-48e4-b1d8-dfe300e923ff

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