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      Transcriptome Responses of Hygromycin B Resistance Gene-Transformed, Hygromycin B-Adaptive and Wild Nannochloropsis oceanica Strains to Hygromycin B

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          In order to decipher the hygromycin B tolerance and resistance mechanisms of Nannochloropsis oceanica, the transcriptome profiles of a transgenic strain carrying a randomly integrated hygromycin B resistant gene, a hygromycin B-adaptive strain and a wild type strain of N. oceanica were compared by transcriptome sequencing (RNA-seq) without referring to a high quality genome sequence. The results showed that the adaptive strain adapts to the hygromycin B existing environments mainly by intensifying the expressions of the efflux pump ABC and MFS superfamily transporter genes, thus reducing the intracellular concentration of hygromycin B. The transgenic strain obtains the hygromycin B resistance ability solely by expressing exogenous resistance gene. Accordingly, the screening and maintenance of N. oceanica transformants should be carried out at an antibiotics concentration higher than the adaptive threshold. Our findings can help the genetic modification of N. oceanica with the application of hygromycin B.

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          Author and article information

          Journal of Ocean University of China
          Science Press and Springer (China )
          24 January 2020
          01 April 2020
          : 19
          : 2
          : 453-458
          1College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
          2Laboratory of Applied Microalgae, College of Fisheries, Ocean University of China, Qingdao 266003, China
          3Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China
          4Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China
          Author notes
          *Corresponding author: GUO Li, E-mail: qdguoli@ ; YANG Guanpin, E-mail: yguanpin@
          Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

          The copyright to this article, including any graphic elements therein (e.g. illustrations, charts, moving images), is hereby assigned for good and valuable consideration to the editorial office of Journal of Ocean University of China, Science Press and Springer effective if and when the article is accepted for publication and to the extent assignable if assignability is restricted for by applicable law or regulations (e.g. for U.S. government or crown employees).

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