Transcriptome Responses of Hygromycin B Resistance Gene-Transformed, Hygromycin B-Adaptive and Wild Nannochloropsis oceanica Strains to Hygromycin B

Abstract

In order to decipher the hygromycin B tolerance and resistance mechanisms of Nannochloropsis oceanica, the transcriptome profiles of a transgenic strain carrying a randomly integrated hygromycin B resistant gene, a hygromycin B-adaptive strain and a wild type strain of N. oceanica were compared by transcriptome sequencing (RNA-seq) without referring to a high quality genome sequence. The results showed that the adaptive strain adapts to the hygromycin B existing environments mainly by intensifying the expressions of the efflux pump ABC and MFS superfamily transporter genes, thus reducing the intracellular concentration of hygromycin B. The transgenic strain obtains the hygromycin B resistance ability solely by expressing exogenous resistance gene. Accordingly, the screening and maintenance of N. oceanica transformants should be carried out at an antibiotics concentration higher than the adaptive threshold. Our findings can help the genetic modification of N. oceanica with the application of hygromycin B.

Journal

Journal ID (publisher-id): JOUC

Title: Journal of Ocean University of China

Publisher: Science Press and Springer (China )

ISSN (Electronic): 1672-5182

Publication date (Electronic): 24 January 2020

Publication date (Print): 01 April 2020

Volume: 19

Issue: 2

Pages: 453-458

Affiliations

¹College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China

²Laboratory of Applied Microalgae, College of Fisheries, Ocean University of China, Qingdao 266003, China

³Institutes of Evolution and Marine Biodiversity, Ocean University of China, Qingdao 266003, China

⁴Key Laboratory of Marine Genetics and Breeding of Ministry of Education, Ocean University of China, Qingdao 266003, China

Author notes

*Corresponding author: GUO Li, E-mail: qdguoli@ 123456139.com ; YANG Guanpin, E-mail: yguanpin@ 123456ouc.edu.cn

Article

Publisher ID: s11802-020-4252-4

DOI: 10.1007/s11802-020-4252-4

Copyright statement: Copyright © Ocean University of China, Science Press and Springer-Verlag GmbH Germany 2020.

License:

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Product

Self URI (journal-page): https://www.springer.com/journal/11802