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      A more efficient method to generate integration-free human iPS cells.

      Nature Methods

      Asian Continental Ancestry Group, Tissue Donors, genetics, Plasmids, metabolism, immunology, cytology, Induced Pluripotent Stem Cells, Humans, HLA Antigens, Genetic Vectors, Gene Frequency, Gene Expression Profiling, Electroporation, methods, Cell Culture Techniques

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          Abstract

          We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCs) with episomal plasmid vectors. We generated human iPSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match ∼20% of the Japanese population at major HLA loci; most iPSCs are integrated transgene-free. This method may provide iPSCs suitable for autologous and allologous stem-cell therapy in the future.

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          Journal
          10.1038/nmeth.1591
          21460823

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