8
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      New methods for detection of campylobacters in stool samples in comparison to culture.

      Journal of Clinical Microbiology
      Adult, Bacteriological Techniques, methods, Campylobacter Infections, diagnosis, Campylobacter coli, genetics, growth & development, isolation & purification, Campylobacter jejuni, Child, Child, Preschool, Feces, microbiology, Female, Humans, Infant, Infant, Newborn, Male, Molecular Diagnostic Techniques, Sensitivity and Specificity

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Campylobacter species, especially Campylobacter jejuni and Campylobacter coli, are a major cause of human bacterial enteritis. Current detection in stools is done essentially by culture on selective and nonselective media with filtration. These methods were compared to 2 molecular biology methods, an in-house real-time PCR and a multiplex PCR named Seeplex Diarrhea ACE Detection, and 3 immunoenzymatic methods, Premier Campy, RidaScreen Campylobacter, and ImmunoCard Stat!Campy. Out of 242 stool specimens tested, 23 (9.5%) fulfilled the positivity criteria, i.e., they were positive by one or both culture methods or, in case of a negative culture, by a positive molecular method and a positive immunoenzymatic method. The striking feature of this study is the low sensitivity of culture, in the range of 60%, in contrast to immunoenzymatic and molecular tests.

          Related collections

          Author and article information

          Comments

          Comment on this article