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      Diversidad genética de accesiones de nacedero Trichanthera gigantea (Humb. & Bonpl.) Nees, mediante RAM's (Random Amplified Microsatellites) Translated title: Genetic diversity of accessions of Trichanthera gigantea (Humb. & Bonpl.) Nees, using RAMs (Random Amplified Microsatellites)

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          Abstract

          Con el objeto de estudiar la diversidad genética del árbol forrajero nacedero Trichanthera gigantea (Humb. Bonpl.) Nees, existente en la colección nacional de la Fundación Centro para la Investigación en Sistemas Sostenibles de Producción Agropecuaria (CIPAV) se utilizaron cinco cebadores RAMs en 38 accesiones procedentes de once departamentos de Colombia y dos estados de Venezuela. Se obtuvieron 71 bandas usadas para estimar los parámetros de diversidad genética. Se adaptó un protocolo de extracción de ADN para la especie y se estableció un banco de ADN de las accesiones en la Universidad Nacional de Colombia sede Palmira, para la realización de trabajos futuros. Los resultados indicaron que la colección nacional de nacedero presenta heterogeneidad en sus accesiones, alta diversidad genética y no se encontró relación entre los grupos genéticos formados mediante el análisis molecular y las zonas geográficas de origen

          Translated abstract

          To study the genetic diversity of the national collection of Trichanthera gigantea (Humb. & Bonpl.) Nees from the Foundation Center for Research in Sustainable Systems for Agricultural Production (CIPAV), five primers RAMs were used on 38 accessions from 11 departments of Colombia and two states of Venezuela. A pattern of 71 bands was obtained and used to estimate genetic diversity parameters. A specific protocol for DNA isolation was adapted and a DNA bank was established at the National University of Colombia, Palmira campus for further investigations. The results showed heterogeneity and high genetic diversity in these accessions. No relationship was found among molecular group analysis and the geographical origin zones.

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          Most cited references26

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          Estimation of average heterozygosity and genetic distance from a small number of individuals.

          M Nei (1978)
          The magnitudes of the systematic biases involved in sample heterozygosity and sample genetic distances are evaluated, and formulae for obtaining unbiased estimates of average heterozygosity and genetic distance are developed. It is also shown that the number of individuals to be used for estimating average heterozygosity can be very small if a large number of loci are studied and the average heterozygosity is low. The number of individuals to be used for estimating genetic distance can also be very small if the genetic distance is large and the average heterozygosity of the two species compared is low.
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            A plant DNA minipreparation: Version II

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              Length polymorphisms of simple sequence repeat DNA in soybean.

              The objective of this work was to ascertain the presence and degree of simple sequence repeat (SSR) DNA length polymorphism in the soybean [Glycine max (L.) Merr.]. A search of GenBank revealed no (CA)n or (GT)n SSRs with n greater than 8 in soybean. In contrast, 5 (AT)n and 1 (ATT)n SSRs with n ranging from 14 to 27 were detected. Polymerase chain reaction (PCR) primers to regions flanking the six SSR loci were used in PCR amplification of DNA from 43 homozygous soybean genotypes. At three loci, amplification produced one PCR product per genotype and revealed 6, 7 and 8 product length variants (alleles) at the three loci, respectively. F1 hybrids between parents carrying different alleles produced two PCR products identical to the two parents. Codominant segregation of alleles among F2 progeny was demonstrated at each locus. A soybean DNA library was screened for the presence of (CA/GT)n SSRs. Sequencing of positive clones revealed that the longest such SSR was (CA)9. Thus, (CA)n SSRs with n of 15 or more are apparently much less common in soybean than in the human genome. In contrast to humans, (CA)n SSRs will probably not provide an abundant source of genetic markers in soybean. However, the apparent abundance of long (AT)n sequences should allow this SSR to serve as a source of highly polymorphic genetic markers in soybean.
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                Author and article information

                Journal
                acag
                Acta Agronómica
                Acta Agron.
                Universidad Nacional de Colombia (Palmira, Valle, Colombia )
                0120-2812
                April 2011
                : 60
                : 2
                : 120-131
                Affiliations
                [03] Cali Valle del Cauca orgnameFundación Centro para la Investigación en Sistemas Sostenibles de Producción Agropecuaria (CIPAV) Colombia ampossotunal.edu.co, ampossot@ 123456gmail.com
                [02] Santiago de Cali Valle del Cauca orgnameUniversidad del Valle orgdiv1Departamento de Biología Colombia
                [01] Palmira, Valle del Cauca orgnameUniversidad Nacional de Colombia sede Palmira orgdiv1Facultad de Ciencias Agropecuarias Colombia
                Article
                S0120-28122011000200003 S0120-2812(11)06000203
                f7a7d85a-b265-4947-bc23-0c2f271bab17

                This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

                History
                : 05 May 2011
                : 09 September 2011
                Page count
                Figures: 0, Tables: 0, Equations: 0, References: 33, Pages: 12
                Product

                SciELO Colombia

                Self URI: Texto completo solamente en formato PDF (ES)
                Categories
                Articulos Originales

                nacedero,marcadores genéticos,extracción de ADN,bancos de germoplasma,ADN,Trichanthera gigantea,germplasm banks,genetic markers,DNA isolation,DNA

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