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Dispositions of di- and mono-(2-ethylhexyl) phthalate in newborn infants subjected to exchange transfusions

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      Studies on the hepatic effects of orally administered di-(2-ethylhexyl) phthalate in the rat

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        Effect of some phthalate esters and other testicular toxins on primary cultures of testicular cells.

        Mixed cultures of Sertoli and germ cells were prepared from rat testes and their response to some model testicular toxins was studied. Cultures consisted of a monolayer of Sertoli cells to which clusters of spermatocytes and spermatogonia adhered. With time in culture, germ cells progressively detached from the Sertoli cells into the medium. Addition of mono-(2-ethylhexyl) phthalate (MEHP) to the culture medium resulted in a concentration-dependent increase in the rate of germ-cell detachment over the range 10(-7) - 10(-4) M. No such effect was produced by di-(2-ethylhexyl) phthalate or 2-ethylhexanol. An increased rate of germ-cell detachment was also produced by other phthalate monoesters known to cause testicular damage in vivo, whereas similar concentrations of a number of monophthalates not known to affect the testis in vivo had no such effect on the cultures. Known age and species differences in the testicular toxicity of di-(2-ethylhexyl) phthalate could be reproduced in cultures treated with MEHP. There was little effect on the viability of either germ cells or Sertoli cells at concentrations of MEHP that caused marked germ-cell detachment, but there were changes in Sertoli-cell morphology. Increased germ-cell detachment was also observed in cultures treated with 10(-7) - 10(-4) M-AF1312/TS, a compound that affects Sertoli cells in vivo, but was not seen in cultures exposed to a range of other testicular toxins with different target cells in the testis. Thus, the effects produced by phthalate monoesters in vitro may reflect damage to the Sertoli cells. Testicular cell cultures could be of value both for screening compounds and for studying underlying mechanisms of testicular toxicity.
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          Testicular effects of phthalate esters.

           S Gangolli (1982)
          The testicular effects produced by di(2-ethylhexyl) phthalate (DEHP) in the rat, characterized by a decrease in the relative organ weight and histological changes in the seminiferous tubules, can also be produced by di-n-butyl, di-n-pentyl and di-n-hexyl phthalates. The corresponding monoesters of these compounds, formed in vivo as a result of the action of nonspecific esterases in the intestinal mucosa and other tissues, were equally effective in inducing testicular damage. Phthalate-induced testicular injury was accompanied by a decrease in the zinc content in the gonads and in increased urinary excretion of this element. Exposure of preparations of rat seminiferous tubule cells in culture to monophthalates capable of producing testicular injury resulted in a dose-related detachment of germinal cells from Sertoli cells in a manner similar to the effect seen in the intact animal. This in vitro system may find application in the elucidation of the toxic mechanisms involved in phthalate-induced testicular injury and in screening compounds likely to act in a manner similar to phthalates. Images FIGURE 1. (A) FIGURE 1. (B) FIGURE 2. (A) FIGURE 2. (B) FIGURE 2. (C) FIGURE 2. (D) FIGURE 3. (A) FIGURE 3. (B) FIGURE 4. (A) FIGURE 4. (B)
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            Author and article information

            Journal
            European Journal of Clinical Investigation
            Eur J Clin Invest
            Wiley-Blackwell
            0014-2972
            1365-2362
            December 1985
            December 1985
            : 15
            : 6
            : 430-436
            10.1111/j.1365-2362.1985.tb00297.x
            © 1985

            http://doi.wiley.com/10.1002/tdm_license_1.1

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