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Microbial Metagenomics, Metatranscriptomics, and Metaproteomics
A Metagenomics Portal for a Democratized Sequencing World
edited_book
Author(s):
Andreas Wilke
,
Elizabeth M. Glass
,
Daniela Bartels
,
Jared Bischof
,
Daniel Braithwaite
,
Mark D’Souza
,
Wolfgang Gerlach
,
Travis Harrison
,
Kevin Keegan
,
Hunter Matthews
,
Renzo Kottmann
,
Tobias Paczian
,
Wei Tang
,
William L. Trimble
,
Pelin Yilmaz
,
Jared Wilkening
,
Narayan Desai
,
Folker Meyer
Publication date
(Print):
2013
Publisher:
Elsevier
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Educational Challenges
Author and book information
Book Chapter
Publication date (Print):
2013
Pages
: 487-523
DOI:
10.1016/B978-0-12-407863-5.00022-8
SO-VID:
f7c0f92b-29c6-4271-a7e7-73eacef8c76c
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Book chapters
pp. ii
Series Page
pp. iv
Copyright
pp. xiii
Contributors
pp. xxi
Preface
pp. xxiii
Methods in Enzymology
pp. 3
In-Solution Fluorescence In Situ Hybridization and Fluorescence-Activated Cell Sorting for Single Cell and Population Genome Recovery
pp. 21
Whole Cell Immunomagnetic Enrichment of Environmental Microbial Consortia Using rRNA-Targeted Magneto-FISH
pp. 45
Coupling FACS and Genomic Methods for the Characterization of Uncultivated Symbionts
pp. 61
Optofluidic Cell Selection from Complex Microbial Communities for Single-Genome Analysis
pp. 91
Quantifying and Identifying the Active and Damaged Subsets of Indigenous Microbial Communities
pp. 111
Preparation of BAC Libraries from Marine Microbial Populations
pp. 123
Preparation of Fosmid Libraries and Functional Metagenomic Analysis of Microbial Community DNA
pp. 143
Preparation of Metagenomic Libraries from Naturally Occurring Marine Viruses
pp. 169
Preparation and Metatranscriptomic Analyses of Host–Microbe Systems
pp. 187
Preparation of Microbial Community cDNA for Metatranscriptomic Analysis in Marine Plankton
pp. 219
Sequential Isolation of Metabolites, RNA, DNA, and Proteins from the Same Unique Sample
pp. 237
Use of Internal Standards for Quantitative Metatranscriptome and Metagenome Analysis
pp. 251
Sample Processing and cDNA Preparation for Microbial Metatranscriptomics in Complex Soil Communities
pp. 271
Sample Preparation and Processing for Planktonic Microbial Community Proteomics
pp. 289
Sample Handling and Mass Spectrometry for Microbial Metaproteomic Analyses
pp. 305
Molecular Tools for Investigating Microbial Community Structure and Function in Oxygen-Deficient Marine Waters
pp. 333
Assembling Full-Length rRNA Genes from Short-Read Metagenomic Sequence Datasets Using EMIRGE
pp. 353
Computational Methods for High-Throughput Comparative Analyses of Natural Microbial Communities
pp. 371
Advancing Our Understanding of the Human Microbiome Using QIIME
pp. 445
Disentangling Associated Genomes
pp. 465
Microbial Community Analysis Using MEGAN
pp. 487
A Metagenomics Portal for a Democratized Sequencing World
pp. 525
A User’s Guide to Quantitative and Comparative Analysis of Metagenomic Datasets
pp. 549
Author Index
pp. 581
Subject Index
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