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      Molecular epidemiology of malaria in Cameroon. XXVI. Twelve-year in vitro and molecular surveillance of pyrimethamine resistance and experimental studies to modulate pyrimethamine resistance.

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      The American journal of tropical medicine and hygiene

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          Abstract

          In vitro pyrimethamine response of Plasmodium falciparum isolates and dihydrofolate reductase (dhfr) gene sequences were analyzed in 2004-2005 and compared with our previous data. Most isolates (n = 103, all dhfr mutants) had 50% inhibitory concentrations (IC(50)s) > or = 119 nM, and six isolates had low IC(50)s (five wild-type or mixed dhfr, 0.04-1.37 nM; one triple mutant, 6.4 nM). Of 194 isolates, only 7 had the wild-type dhfr and 187 were mutants. The results of the two methods were highly concordant and indicated a significant increase (P < 0.05) in the prevalence of mutant, pyrimethamine-resistant P. falciparum between 1994 and 2005. The addition of probenecid or sulfinpyrazone to pyrimethamine resulted in a slight-to-moderate decrease in the level of in vitro pyrimethamine resistance without rendering the parasites susceptible to pyrimethamine. Analysis of molecular markers may be useful for the long-term surveillance of antifolate-resistant malaria.

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          Author and article information

          Journal
          Am J Trop Med Hyg
          The American journal of tropical medicine and hygiene
          0002-9637
          0002-9637
          Aug 2007
          : 77
          : 2
          Affiliations
          [1 ] Unité de Recherche 77 Paludologie Afro-Tropicale, Institut de Recherche pour le Développement, Organisation de Coordination pour la lutte contre les Endémies en Afrique Centrale, Yaoundé, Cameroon. rachidatahar@yahoo.fr
          Article
          77/2/221
          10.1016/j.actatropica.2007.04.008
          17690390
          f83f8a01-dfd7-43ca-85bd-a33999509228
          History

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