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Clean-up and re-use of kieselguhr (Extrelut) for liquid-liquid extraction of urinary cortisol.

Journal of chromatography. B, Biomedical sciences and applications

Male, urine, Hydrocortisone, Humans, Diatomaceous Earth, Chromatography, High Pressure Liquid

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      Abstract

      Cortisol was isolated from human urine using kieselguhr (Extrelut)-filled columns. After use, Extrelut was cleaned-up once with distilled water and twice with ethanol. Before re-use, the cleaned-up kieselguhr was dried for 24 h by a warm air stream. The comparison of cortisol recovery from human urine and HPLC chromatograms of urinary extracts show that Extrelut can be repeatedly used for liquid-liquid extraction of urinary cortisol.

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