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      Clean-up and re-use of kieselguhr (Extrelut) for liquid-liquid extraction of urinary cortisol.

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      Journal: Journal of chromatography. B, Biomedical sciences and applications
      Keywords: Chromatography, High Pressure Liquid, Diatomaceous Earth, Humans, Hydrocortisone, urine, Male

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          Abstract

          Cortisol was isolated from human urine using kieselguhr (Extrelut)-filled columns. After use, Extrelut was cleaned-up once with distilled water and twice with ethanol. Before re-use, the cleaned-up kieselguhr was dried for 24 h by a warm air stream. The comparison of cortisol recovery from human urine and HPLC chromatograms of urinary extracts show that Extrelut can be repeatedly used for liquid-liquid extraction of urinary cortisol.

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          PubMed ID:: 9187407

          Keywords: Chromatography, High Pressure Liquid,Diatomaceous Earth,Humans,Hydrocortisone,urine,Male
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          Keywords: Chromatography, High Pressure Liquid, Diatomaceous Earth, Humans, Hydrocortisone, urine, Male

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