Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats

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Abstract

Background

Dietary fish oil (DFO) has been identified as a micronutrient supplement with the potential to improve musculoskeletal health in old age. Few data are available for effects of DFO on muscle contractility, despite the significant negative impact of muscle weakness on age-related health outcomes. Accordingly, the effects of a DFO intervention on the contractile function and proteomic profile of adult and aged in an animal model of aging were investigated.

Methods

This preliminary study evaluated 14 adult (8 months) and 12 aged (22 months) male, Sprague-Dawley rats consuming a DFO-supplemented diet or a control diet for 8 weeks (7 adult and 6 aged/dietary group). Animal weight, food intake and grip strength were assessed at the start and end of the FO intervention. In situ force and contractile properties were measured in the medial gastrocnemius muscle following the intervention and muscles were processed for 2-D gel electrophoresis and proteomic analysis via liquid chromatography with tandem mass spectrometry, confirmed by immunoblotting. Effects of age, diet and age x diet interaction were evaluated by 2-way ANOVA.

Results

A significant ( P = 0.022) main effect for DFO to increase (~ 15%) muscle contractile force was observed, without changes in muscle mass. Proteomic analysis revealed a small number of proteins that differed across age and dietary groups at least 2-fold, most of which related to metabolism and oxidative stress. In seven of these proteins (creatine kinase, triosephosphate isomerase, pyruvate kinase, parvalbumin, beta-enolase, NADH dehydrogenase and Parkin7/DJ1), immunoblotting corroborated these findings. Parvalbumin showed only an effect of diet (increased with DFO) ( P = 0.003). Significant age x diet interactions were observed in the other proteins, generally demonstrating increased expression in adult and decreased expression aged rats consuming DFO (all P > 0.011). However, correlational analyses revealed no significant associations between contractile parameters and protein abundances.

Conclusions

Results of this preliminary study support the hypothesis that DFO can enhance musculoskeletal health in adult and aged muscles, given the observed improvement in contractile function. The fish oil supplement also alters protein expression in an age-specific manner, but the relationship between proteomic and contractile responses remains unclear. Further investigation to better understand the magnitude and mechanisms muscular effects of DFO in aged populations is warranted.

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Most cited references 61

Record: found
Abstract: found
Article: not found

The healthcare costs of sarcopenia in the United States.

Ian Janssen, Donald S. Shepard, Peter Katzmarzyk … (2004)

To estimate the healthcare costs of sarcopenia in the United States and to examine the effect that a reduced sarcopenia prevalence would have on healthcare expenditures. Cross-sectional surveys. Nationally representative surveys using data from the U.S. Census, Third National Health and Nutrition Examination Survey, and National Medical Care and Utilization Expenditure Survey. Representative samples of U.S. adults aged 60 and older. The healthcare costs of sarcopenia were estimated based on the effect of sarcopenia on increasing physical disability risk in older persons. In the first step, the healthcare cost of disability in older Americans was estimated from national surveys. In the second step, the proportion of the disability cost due to sarcopenia (population-attributable risk) was calculated to determine the healthcare costs of sarcopenia. These calculations relied upon previously published relative risk values for disability in sarcopenic individuals and sarcopenia prevalence rates in the older population. The estimated direct healthcare cost attributable to sarcopenia in the United States in 2000 was $18.5 billion ($10.8 billion in men, $7.7 billion in women), which represented about 1.5% of total healthcare expenditures for that year. A sensitivity analysis indicated that the costs could be as low as $11.8 billion and as high as $26.2 billion. The excess healthcare expenditures were $860 for every sarcopenic man and $933 for every sarcopenic woman. A 10% reduction in sarcopenia prevalence would result in savings of $1.1 billion (dollars adjusted to 2000 rate) per year in U.S. healthcare costs. Sarcopenia imposes a significant but modifiable economic burden on government-reimbursed healthcare services in the United States. Because the number of older Americans is increasing, the economic costs of sarcopenia will escalate unless effective public health campaigns aimed at reducing the occurrence of sarcopenia are implemented.

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Record: found
Abstract: found
Article: not found

The relationship between leg power and physical performance in mobility-limited older people.

Terence Herman, Kelly Mizer, Suzanne G Leveille … (2002)

The purpose of this study was to assess the influence of leg power and leg strength on the physical performance of community-dwelling mobility-limited older people. Cross-sectional analysis of baseline data from a 12-week randomized controlled exercise-intervention study. Exercise laboratory within the Department of Health Science of an urban university. Forty-five community-dwelling mobility-limited people (34 women, 11 men), aged 65 to 83. Health status, depression, cognition, physical activity, and falls efficacy; physiological measures of lower extremity strength and power; and measures of physical performance. Through bivariate analyses, leg power was significantly associated with physical performance as measured by stair-climb time, chair-stand time, tandem gait, habitual gait, maximal gait, and the short physical performance battery describing between 12% and 45% of the variance (R2). Although leg power and leg strength were greatly correlated (r = .89) in a comparison of bivariate analyses of strength or power with physical performance, leg power modeled up to 8% more of the variance for five of six physical performance measures. Despite limitations in sample size, it appeared that, through quadratic modeling,the influence of leg power on physical performance was curvilinear. Using separate multivariate analyses, partial R2 values for leg power and leg strength were compared, demonstrating that leg power accounted for 2% to 8% more of the variance with all measures of physical performance. Leg power is an important factor influencing the physical performance of mobility-limited older people. Although related to strength, it is a separate attribute that may exert a greater influence on physical performance. These findings have important implications for clinicians practicing geriatric rehabilitation.

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Initial manifestations of frailty criteria and the development of frailty phenotype in the Women's Health and Aging Study II.

Jacquelyn L. Fried, Karen Bandeen-Roche, Qian Xue … (2008)

Understanding points of onset of the frailty syndrome is vital to early identification of at-risk individuals and to targeting intervention efforts to those components that are first affected, when reversal may be most possible. This study aims to characterize natural history by which commonly used frailty criteria manifest and to assess whether the rate of progression to frailty depends on initial manifestations. The investigation was based on a 7.5-year observational study of 420 community-dwelling women aged 70-79 years who were not frail at baseline, with frailty defined as meeting>or=3 of 5 criteria: weight loss, slow walking speed, weakness, exhaustion, and low physical activity level. The 7.5-year incidence of frailty was 9% among women who were nonfrail at baseline. Despite significant heterogeneity, weakness was the most common first manifestation, and occurrence of weakness, slowness, and low physical activity preceded exhaustion and weight loss in 76% of the women who were nonfrail at baseline. Women with exhaustion or weight loss as initial presenting symptoms were 3-5 times more likely to become frail than were women without any criterion (p<.05). Our findings suggest that weakness may serve as a warning sign of increasing vulnerability in early frailty development, and weight loss and exhaustion may help to identify women most at risk for rapid adverse progression.

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Author and article information

Contributors

David W. Russ:

ORCID: http://orcid.org/0000-0002-8744-1399

Druss2@usf.edu

Journal

Journal ID (nlm-ta): Lipids Health Dis

Journal ID (iso-abbrev): Lipids Health Dis

Title: Lipids in Health and Disease

Publisher: BioMed Central (London )

ISSN (Electronic): 1476-511X

Publication date (Electronic): 9 July 2020

Publication date PMC-release: 9 July 2020

Publication date Collection: 2020

Volume: 19

Electronic Location Identifier: 165

Affiliations

[1 ]GRID grid.170693.a, ISNI 0000 0001 2353 285X, School of Physical Therapy & Rehabilitation Sciences, , University of South Florida, ; 12901 Bruce B. Downs Blvd., Tampa, FL MDC77 USA

[2 ]GRID grid.20627.31, ISNI 0000 0001 0668 7841, Ohio Musculoskeletal and Neurological Institute (OMNI), , Heritage College of Osteopathic Medicine, ; Athens, OH USA

[3 ]GRID grid.263724.6, ISNI 0000 0001 1945 4190, Center for Proteomics, , Smith College, ; Northampton, MA USA

[4 ]GRID grid.263724.6, ISNI 0000 0001 1945 4190, Program in Biochemistry, , Smith College, ; Northampton, MA USA

[5 ]GRID grid.417574.4, ISNI 0000 0004 0366 7505, Abbott Nutrition R&D, ; 3300 Stelzer Road, Columbus, OH USA

[6 ]Present address: BIO-CAT, 9117 3 Notch Rd, Troy, VA 22974 USA

Author information

David W. Russ http://orcid.org/0000-0002-8744-1399

Article

Publisher ID: 1333

DOI: 10.1186/s12944-020-01333-4

PMC ID: 7350698

PubMed ID: 32646455

SO-VID: f8792568-22ef-4e0f-955e-809439c80458

License:

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

History

Date received : 23 March 2020

Date accepted : 18 June 2020

Custom metadata

ScienceOpen disciplines: Biochemistry

Keywords: skeletal muscle,sarcopenia,proteomics,diet,omega-3,aging

Data availability:

ScienceOpen disciplines: Biochemistry

Keywords: skeletal muscle, sarcopenia, proteomics, diet, omega-3, aging

Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats

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Most cited references 61

The healthcare costs of sarcopenia in the United States.

The relationship between leg power and physical performance in mobility-limited older people.

Initial manifestations of frailty criteria and the development of frailty phenotype in the Women's Health and Aging Study II.

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