Characterization and Screening of Thermophilic  Bacillus  Strains for Developing Plant Growth Promoting Consortium From Hot Spring of Leh and Ladakh Region of India

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Abstract

In the present investigation, the main aim is to identify and characterize the potential drought tolerant plant growth promoting consortium for agricultural productivity. Three bacterial isolates were isolated from hot spring of Chumathang area of Leh district. Bacillus species (BHUJP-H1, BHUJP-H2, and BHUJP-H3) were done some biochemical tests including catalase, cellulase, amylase, indole-3-acetic acid, phosphate solubilisation, production of ammonia, siderophore, and hydrogen cyanide. Molecular characterization of isolates was done by 16S rDNA sequencing, e.g., Bacillus subtilis BHUJP-H1 (KU312403), Bacillus sp. BHUJP-H2 (KU312404) and B. licheniformis BHUJP-H3 (KU312405). The genetic diversity of the isolates was assessed by seven inter simple sequence repeat, all primer shows high polymorphism. The highest polymorphism efficiency and polymorphism information content showed by UBC-809 and UBC-836 which were 100% and 0.44 respectively, the lowest is by UBC-807 75% and 0.28 respectively. On an average 90.69% polymorphism efficiency and 0.40 polymorphism information contents obtained by used markers. The highest, 11.08 and the lowest, 4.50 effective multiplex ratios obtained for primer UBC-823 and UBC-807, on an average 7.99 effective multiplex ratio obtained. The highest, 4.89 and the lowest, 1.25 marker indexes obtained by UBC-836 and UBC-807 respectively and on an average 3.24 obtained. The UPGMA cluster analysis divided a population into two clusters I and II, in which BHUJP-H1 and BHUJP-H2 grouped under same while BHUJP-H3 grouped under another cluster. The treatment combination of Bacillus subtilis BHUJP-H1, B. subtilis BHUJP-H1+ B. licheniformis BHUJP-H3 and B. subtilis BHUJP-H1+ Bacillus sp. BHUJP-H2+ B. licheniformis BHUJP-H3 were recorded better combination for enhancing plant growth attributes of Vigna radiata as compared to control and others. The plant growth promoting consortium, e.g., Bacillus subtilis BHUJP-H1, Bacillus subtilis BHUJP-H1+ B. licheniformis BHUJP-H3 and B. subtilis BHUJP-H1+ Bacillus sp. BHUJP-H2+ B. licheniformis BHUJP-H3 can be further used as effective microbial inoculant for enhancing the production of mungbean in field conditions. Bacillus sp. BHUJP-H1 and Bacillus sp. BHUJP-H2 may use as drought tolerant plant growth promoting consortium for enhancing the sustainable agricultural productivity.

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Author and article information

Contributors

Jay Prakash Verma: URI : http://loop.frontiersin.org/people/244641/overview

Durgesh Kumar Jaiswal: URI : http://loop.frontiersin.org/people/305782/overview

Journal

Journal ID (nlm-ta): Front Microbiol

Journal ID (iso-abbrev): Front Microbiol

Journal ID (publisher-id): Front. Microbiol.

Title: Frontiers in Microbiology

Publisher: Frontiers Media S.A.

ISSN (Electronic): 1664-302X

Publication date (Electronic): 26 June 2018

Publication date Collection: 2018

Volume: 9

Electronic Location Identifier: 1293

Affiliations

[1] ¹Institute of Environment and Sustainable Development, Banaras Hindu University , Varanasi, India

[2] ²Hawkesbury Institute for the Environment, Western Sydney University , Sydney, NSW, Australia

[3] ³Department of Soil Science and Agricultural Chemistry, Institute of Agricultural Sciences, Banaras Hindu University , Varanasi, India

[4] ⁴Synthetic Biology Laboratory, Department of Microbiology, School of Biological Sciences and Biotechnology, Institute of Advanced Research , Gandhinagar, India

Author notes

Edited by: Giuseppe Colla, Università degli Studi della Tuscia, Italy

Reviewed by: Ömür Baysal, Muǧla University, Turkey; Giridhar Anam Babu, Chonbuk National University, South Korea

*Correspondence: Jay Prakash Verma, verma_bhu@ 123456yahoo.co.in ; jpv.iesd@ 123456bhu.ac.in

This article was submitted to Plant Microbe Interactions, a section of the journal Frontiers in Microbiology

Article

DOI: 10.3389/fmicb.2018.01293

PMC ID: 6028593

PubMed ID: 29997578

SO-VID: f8c5a6a6-8213-495e-b59d-59f6f5782741

License:

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

History

Date received : 03 August 2017

Date accepted : 28 May 2018

Page count

Figures: 5, Tables: 7, Equations: 0, References: 80, Pages: 15, Words: 0

Funding

Funded by: Council of Scientific and Industrial Research 10.13039/501100001412

Award ID: P-25/360

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Characterization and Screening of Thermophilic Bacillus Strains for Developing Plant Growth Promoting Consortium From Hot Spring of Leh and Ladakh Region of India

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