hCG Responsiveness of Purified Leydig Cells from Immature and Mature Rats

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Abstract

Collagenase dispersed cells from immature or mature rat testis were separated into 11 bands on a discontinuous Percoll gradient. Erythrocytes sedimented at the bottom. Only three bands (8, 9 and 10) bind efficiently [<sup>125</sup>I]hCG. In immature rats, cells in bands 8, 9 and 10 bind hCG with the same affinity, but differ slightly in their total number of sites (average 65,000 ± 4,000 sites per Leydig cell) and in their cAMP and testosterone production in response to hCG. They present 8 % of the total number of dispersed cells. In mature rat testis, populations 8, 9 and 10 differed markedly in their capacity to bind [<sup>125</sup>I]hCG and in their response to hCG as regards an increase in cAMP and testosterone production. Testosterone production was increased 2- and 17-fold in bands 8 and 10, respectively. In mature rats only 1.2% of the total cells are Leydig cells. In both mature and immature rats, band 10 is made of 100% Leydig cells, as all cells stained positively for 3β-hydroxysteroid dehydrogenase.

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Author and article information

Journal

Journal ID (publisher-id): HRE

Journal ID (nlm-ta): Horm Res Paediatr

Journal ID (doi): 10.1159/issn.1663-2818

Title: Hormone Research in Paediatrics

Publisher: S. Karger AG

ISSN (Print): 1663-2818

ISSN (Electronic): 1663-2826

Publication date Subscription-year: 1983

Publication date (Print): 1983

Publication date (Electronic): 26 November 2008

Volume: 17

Issue: 2

Pages: 114-120

Affiliations

Unité de Recherches sur le Contrôle Hormonal des Activités Cellulaires, Inserm U-162, Hôpital Debrousse, Lyon, France

Article

Publisher ID: 179685 Other ID: Horm Res 1983;17:114–120

DOI: 10.1159/000179685

PubMed ID: 6301963

SO-VID: f93498e7-6236-447e-941c-b2b574a6ab3e

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 28 January 1982

Date accepted : 27 May 1982

Page count

Pages: 7

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