+1 Recommend
0 collections
      • Record: found
      • Abstract: found
      • Article: not found

      Effects of aerobic training on pyruvate dehydrogenase and pyruvate dehydrogenase kinase in human skeletal muscle.

      The Journal of Physiology

      Adaptation, Physiological, Adult, Bicycling, physiology, Biopsy, Needle, Carbohydrate Metabolism, Citrate (si)-Synthase, biosynthesis, Electron Transport Complex II, Electron Transport Complex IV, Exercise, Gene Expression Regulation, Enzymologic, Humans, Male, Mitochondria, Muscle, enzymology, Muscle, Skeletal, Protein Kinases, genetics, metabolism, Protein Subunits, Time Factors, Protein-Serine-Threonine Kinases, Pyruvate Dehydrogenase Complex, RNA, Messenger, analysis

      Read this article at

          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.


          This study examined the effects of short- and long-term aerobic training on the stable up-regulation of pyruvate dehydrogenase (PDH) and PDH kinase (PDK) in human skeletal muscle. We hypothesized that 8 weeks, but not 1 week, of aerobic training would increase total PDH (PDHt) and PDK activities compared to pretraining, and this would be detectable at the level of gene transcription (mRNA) and/or gene translation (protein). Resting muscle biopsies were taken before and after 1 and 8 weeks of aerobic cycle exercise training. PDHt and PDK activities, and their respective protein and mRNA expression, did not differ after 1 week of aerobic training. PDHt activity increased 31% after 8 weeks and this may be partially due to a 1.3-fold increase in PDH-E(1)alpha protein expression. PDK activity approximately doubled after 8 weeks of aerobic training and this was attributed to a 1.3-fold increase in PDK2 isoform protein expression. Similar to 1 week, no changes were observed at the mRNA level after 8 weeks of training. These findings suggest that aerobically trained human skeletal muscle has an increased maximal capacity to utilize carbohydrates, evident by increased PDHt, but increased metabolic control sensitivity to pyruvate through increased contribution of PDK2 to total PDK activity.

          Related collections

          Author and article information



          Comment on this article