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      Recovery of Membrane Potentials from Diamide-induced Depolarization in Frog Lens Fibers


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          Measurement of membrane potentials in isolated frog lens fibers was made by means of intracellular microelectrode techniques. The membrane potentials of lens fibers were depolarized to various degrees after exposure to diamide, an -SH inhibitor. When the degree of diamide-induced depolarization was less than 20 mV, the membrane potentials almost fully recovered to the control level within 12 h after immersion in a Ringer’s solution containing dithiothreitol (DTT), a -SH protector. A similar tendency was also recognized in some lenses (57%) whose depolarization was 30 mV. When the degree of depolarization was 40 mV, the membrane potentials further depolarized in all cases tested in spite of treatment with DTT. From this study, it is considered that frog lens fibers could not recover their function if the damage was so severe as to produce a membrane depolarization of more 40 mV. Determination of ionic concentrations in lens fibers revealed a highly significant correlation between the degree of diamide-induced depolarization and changes in concentration ratio of Na<sup>+</sup>/K<sup>+</sup>.

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          Author and article information

          Ophthalmic Res
          Ophthalmic Research
          S. Karger AG
          04 December 2009
          : 15
          : 6
          : 307-313
          Department of Ophthalmology, Kumamoto University Medical School, Kumamoto, Japan
          265277 Ophthalmic Res 1983;15:307–313
          © 1983 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          : 21 February 1983
          : 07 July 1983
          Page count
          Pages: 7
          Original Paper

          Vision sciences,Ophthalmology & Optometry,Pathology
          Frog lens fiber,Intracellular microelectrode,Dithiothreitol,Depolarization,Diamide


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