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      Derivation of attenuated porcine epidemic diarrhea virus (PEDV) as vaccine candidate

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          Abstract

          The field isolate of porcine epidemic diarrhea virus (PEDV) was serially passaged in Vero cells. The cell passaged PEDV, designated KPEDV-9, was tested for its pathogenicity in the neonatal pigs, immunogenicity and safety in the pregnant sows. The result indicated that KPEDV-9 at the 93rd passage revealed reduced pathogenicity in the neonatal pigs. Pregnant sows inoculated with the attenuated virus showed increased immune responses by ELISA. In addition, delivered piglets were protected from challenge of wild type PEDV. The safety test in pregnant sows indicated that all inoculated animals farrowed the average numbers of litters of piglets. The results of this study supported that the attenuated virus derived from serial passage could be applied as vaccine for protecting suckling piglets against PEDV infection.

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          An apparently new syndrome of porcine epidemic diarrhoea.

          Marie Wood (1977)
          Article 0 comments Cited 162 times – based on 0 reviews     Review now
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            Propagation of the virus of porcine epidemic diarrhea in cell culture.

            M. Hofmann, R Wyler (1988)
            Porcine epidemic diarrhea virus (PEDV) was adapted to serial propagation in Vero cell cultures by adding trypsin to the medium. PEDV-infected cells showed a distinct cytoplasmic fluorescence when examined by a fluorescent-antibody-staining technique. Cytopathic effects, such as vacuolation, formation of syncytia, and fusion of cells, were detected even at passage 1 of the PEDV in Vero cells. Once adapted, the virus induced numerous syncytia containing over 100 nuclei. From virus passage 5 on, all cells forming the monolayer were fused and totally destroyed within 24 h after inoculation. Cell culture-grown PEDV had typical coronavirus morphology when viewed by electron microscopy. Attempts to propagate PEDV in several primary and secondary fetal porcine cell cultures in the presence or absence of trypsin were unsuccessful.
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              Experimental infection of pigs with a new porcine enteric coronavirus, CV 777.

              P Debouck, M Pensaert (1980)
              Cesarean-derived colostrum-deprived and conventionally reared pigs were orally inoculated with the coronavirus-like agent, CV 777, isolate from an outbreak of epizootic diarrhea in swine of all ages. Viral particles detected by electron microscopy in the feces and intestinal contents of inoculated pigs had the typical coronavirus morphology. The present studies provided further evidence that this coronavirus-like agent is different from the two known porcine coronaviruses, transmissible gastroenteritis virus and hemagglutinating encephalomyelitis virus. The experimental infection of pigs with this new agent resulted in vomiting, diarrhea, and dehydration. This coronavirus-like agent was shown to replicate in the epithelial cells covering the small intestinal villi but, unlike transmissible gastroenteritis virus, it also replicated in the epithelial cells covering the large intestinal villi.
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                Author and article information

                Journal
                Journal ID (nlm-ta): Vaccine
                Journal ID (iso-abbrev): Vaccine
                Title: Vaccine
                Publisher: Elsevier Science Ltd.
                ISSN (Print): 0264-410X
                ISSN (Electronic): 1873-2518
                Publication date PMC-release: 4 June 1999
                Publication date (Print): 4 June 1999
                Publication date (Electronic): 4 June 1999
                Volume: 17
                Issue: 20
                Pages: 2546-2553
                Affiliations
                [a ]National Veterinary Research and Quarantine Service, 480 Anyang, South Korea
                [b ]Dangjin Livestock Cooperation, 549 Dangjin, South Korea
                Author notes
                [* ]Corresponding author. Tel.: +82-343-467-1860; fax +82-343-449-5882
                Article
                Publisher ID: S0264-410X(99)00059-6
                DOI: 10.1016/S0264-410X(99)00059-6
                PMC ID: 7125954
                PubMed ID: 10418901
                SO-VID: f970b1dd-f0c6-4bde-8b0c-d62fa6f810eb
                Copyright statement: Copyright © 1999 Elsevier Science Ltd. All rights reserved.
                License:

                Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

                History
                Date received : 19 October 1998
                Date revision received : 15 January 1999
                Date accepted : 9 February 1999
                Categories
                Subject: Article

                ScienceOpen disciplines: Infectious disease & Microbiology
                Keywords: porcine epidemic diarrhea virus,attenuation,vaccine candidate
                Data availability:
                ScienceOpen disciplines: Infectious disease & Microbiology
                Keywords: porcine epidemic diarrhea virus, attenuation, vaccine candidate

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