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      Hemolysate Inhibits L-Type Ca 2+ Channels in Rat Basilar Smooth Muscle Cells


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          Erythrocyte lysate increases intracellular Ca<sup>2+</sup> ([Ca<sup>2+</sup>]<sub>i</sub>)and contracts cerebral arteries in vitro and has been suggested to be the cause for cerebral vasospasm. We investigated the effect of hemolysate on L-type Ca<sup>2+</sup> channels directly by using patch clamp techniques in freshly isolated single smooth muscle cells from rat basilar artery. Patch clamp studies revealed a whole-cell current which resembles the L-type Ca<sup>2+</sup> current reported by others. Hemolysate reduced the amplitude of the L-type Ca<sup>2+</sup> channel current. The effect of hemolysate was reversible by washout and repeatable. Hemolysate was separated into two fractions by using filter membranes. The fraction > 1 kDa which contains oxyhemoglobin and other proteins mimicked the effect of hemolysate, while the fraction < 1 kDa and ATP were without effect. We conclude that hemolysate does not increase [Ca<sup>2+</sup>]<sub>i</sub> by activation of L-type Ca<sup>2+</sup> channel.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          24 September 2008
          : 33
          : 3
          : 258-264
          Section of Neurosurgery, Department of Surgery, University of Chicago, 111., USA
          159153 J Vasc Res 1996;33:258–264
          © 1996 S. Karger AG, Basel

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          Pages: 7
          Research Paper


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