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      In vitro Staphylococcus epidermidis Growth in Some Viscoelastic Substances Containing Sodium Hyaluronate

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          The aim of our study was to verify the in vitro growth of Staphylococcus epidermidis in various dilutions of some viscoelastic substances containing hyaluronic acid (Healon® and Healon GV, IAL®, Biolon<sup>TM</sup>). Serial twofold dilutions of each sterile viscoelastic substance, prepared so as to obtain a final concentration ranging from 50 to 0.78% of the product in sterile saline solution (0.85% NaCl), were taken out with a pipette that delivered 1.0 ml/tube. One hundred microliters of the S. epidermidis inocula, used for the evaluation of the positive control of the test organism, was dispensed into each tube. After 24 h of aerobic incubation at 37 °C, 100 μl of sample was taken out from each tube and plated into the specific medium for the growth of the test organism. After 24 h of incubation at 37 °C, these agar plates were examined and the colony-forming unit count of the test organism was compared to the corresponding total colony count, acting as a positive control, in order to determine the quantitative variation of the test organism grown in the presence of the viscoelastic compounds. For the lowest dilutions (from 1: 2 to 1: 8) statistically significant bacterial growth was detected in all tested viscoelastic substances. For the highest dilutions (1: 64 and 1: 128) Biolon and Healon GV showed a significant inhibition of S. epidermidis growth. A significant inhibition was also observed in the highest dilution (1: 128) of Healon. In every dilution of IAL a statistically significant increase in bacterial growth was observed. It remains to be carefully considered whether S. epidermidis, accidentally penetrating the eye via the intraocular lens, could find a culture medium in a small amount of sodium hyaluronate left in the capsular bag behind the optic.

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          Author and article information

          S. Karger AG
          June 1998
          19 March 1998
          : 212
          : 3
          : 184-187
          aInstitute of Ophthalmology, and bInstitute of Experimental Medicine, Chair of Clinical Microbiology, G. D’Annunzio University, Chieti, and cEye Clinic, II University of Naples, Italy
          27274 Ophthalmologica 1998;212:184–187
          © 1998 S. Karger AG, Basel

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          Page count
          Tables: 1, References: 19, Pages: 4
          Original Paper · Travail original · Originalarbeit


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