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      A Plasmodium falciparum GLURP-MSP3 chimeric protein; expression in Lactococcus lactis, immunogenicity and induction of biologically active antibodies.

      Vaccine

      Animals, Antibodies, Protozoan, analysis, biosynthesis, Antigens, Protozoan, chemistry, genetics, immunology, Chromatography, High Pressure Liquid, Enzyme-Linked Immunosorbent Assay, Female, Fermentation, Fluorescent Antibody Technique, Indirect, Immunoglobulin G, Lactococcus lactis, metabolism, Mice, Mice, Inbred BALB C, Plasmids, Plasmodium falciparum, Protozoan Proteins, Recombinant Fusion Proteins, Spectrometry, Mass, Electrospray Ionization, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Vaccines, Synthetic

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          Abstract

          Plasmodium falciparum malaria is a major cause of morbidity and mortality worldwide. To evaluate the efficacy of a possible vaccine antigen against P. falciparum infection, a fusion protein, derived from P. falciparum Glutamate-rich protein (GLURP) genetically coupled to P. falciparum Merozoite surface protein 3 (MSP3) was produced in Lactococcus lactis as a secreted recombinant GLURP-MSP3 fusion protein. The hybrid protein was purified to homogeneity by ion exchange and hydrophobic-interaction chromatography and its composition was verified by MALDI MS, SDS/PAGE and Western blotting with antibodies against antigenic components of GLURP and MSP3. Mice immunized with the hybrid protein produced higher levels of both GLURP- and MSP3-specific antibodies than mice immunized with either GLURP, MSP3 or a mix of both. The protective potential of the hybrid protein was also demonstrated by in vitro parasite-growth inhibition of mouse anti-GLURP-MSP3 IgG antibodies in a monocyte-dependent manner. These results indicate that the GLURP-MSP3 hybrid could be a valuable strategy for future P. falciparum vaccine development.

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          Journal
          15003647
          10.1016/j.vaccine.2003.09.017

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