18
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Myotubularin related protein 7 is essential for the spermatogonial stem cell homeostasis via PI3K/AKT signaling

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          ABSTRACT

          Myotubularin related protein 7 (MTMR7), a key member of the MTMR family, depicts phosphatase activity and is involved in myogenesis and tumor growth. We have previously identified MTMR7 in the proteomic profile of mouse spermatogonial stem cell (SSC) maturation and differentiation, implying that MTMR7 is associated with neonatal testicular development. In this study, to further explore the distribution and function of MTMR7 in mouse testis, we studied the effect of Mtmr7 knockdown on neonatal testicular development by testicular and SSC culture methods. Our results revealed that MTMR7 is exclusively located in early germ cells. Deficiency of MTMR7 by morpholino in neonatal testis caused excessive SSC proliferation, which was attributable to the aberrant PI3K/AKT signaling activation. Altogether, our study demonstrates that MTMR7 maintains SSC homeostasis by inhibiting PI3K/AKT signaling activation.

          Related collections

          Author and article information

          Journal
          Cell Cycle
          Cell Cycle
          KCCY
          kccy20
          Cell Cycle
          Taylor & Francis
          1538-4101
          1551-4005
          2019
          3 September 2019
          : 18
          : 20
          : 2800-2813
          Affiliations
          [a ] Fourth Affiliated Hospital of Jiangsu University , Zhenjiang, China
          [b ] Center for Reproduction and Genetics, Suzhou Municipal Hospital, the Affiliated Suzhou Hospital of Nanjing Medical University , Suzhou, China
          [c ] State Key Laboratory of Reproductive Medicine, Department of Histology and Embryology, Nanjing Medical University , Nanjing, China
          [d ] Center of Clinical Reproductive Medicine, the Affiliated Changzhou Matemity and Child Health Care Hospital of Nanjing Medical University , Changzhou, China
          [e ] The Affiliated Wuxi Matemity and Child Health Care Hospital of Nanjing Medical University , Wuxi, China
          [f ] Reproductive Medicine Center, Northern Jiangsu Province Hospital , Yangzhou, China
          [g ] Department of Obstetrics and Gynecology, Affiliated Hospital of Jiangsu University, Jiangsu University , Zhenjiang, China
          [h ] Human Reproductive and Genetic center, Affiliated Hospital of Jiangnan University , Wuxi, China
          Author notes
          CONTACT Cong Shen 344128944@ 123456qq.com
          [*]

          These authors contributed equally to this work

          Author information
          http://orcid.org/0000-0002-9982-176X
          http://orcid.org/0000-0002-1496-0753
          Article
          PMC6773228 PMC6773228 6773228 1661174
          10.1080/15384101.2019.1661174
          6773228
          31478454
          fa289e6e-54a0-4901-8e0f-b8da7a898162
          © 2019 Informa UK Limited, trading as Taylor & Francis Group
          History
          : 19 May 2019
          : 14 August 2019
          : 21 August 2019
          Page count
          Figures: 8, References: 53, Pages: 14
          Funding
          Funded by: National Natural Science Foundation of China 10.13039/501100001809
          Award ID: 31701298
          Funded by: National Natural Science Foundation of China 10.13039/501100001809
          Award ID: 81601338
          Funded by: Natural Science Foundation of Jiangsu Province 10.13039/501100004608
          Award ID: BK20160177
          Funded by: Natural Science Foundation of Jiangsu Province 10.13039/501100004608
          Award ID: BK20170562
          Funded by: Jiangsu Provincial Medical Innovation Team
          Award ID: CXTDB2017013
          Funded by: Matemity and Child Health Foundation of Jiangsu Province
          Award ID: F201865
          Funded by: Natural Science Foundation of the Jiangsu Higher Education Institutions of China
          Award ID: 16KJB310006
          Funded by: Key Research Foundation of Zhenjiang Social Development
          Award ID: SH2016028
          Funded by: Key Research Foundation of Zhenjiang Social Development
          Award ID: SH2017013
          Funded by: Suzhou Key Laboratory of male reproduction research
          Award ID: SZS201718
          Funded by: Suzhou Introduced Project of Clinical Medical Expert Team
          Award ID: SZYJTD201708
          Funded by: Key Research Foundation of Zhenjiang Social Development
          Award ID: SH2018065
          Funded by: Suzhou Key Medical Center
          Award ID: SZZX201505
          Funded by: Basic Program of Nanjing Medical University
          Award ID: 2017NJMU163
          Funded by: Open Fund of State Key Laboratory of Reproductive Medicine of Nanjing Medical University
          Award ID: SKLRM-KA201704
          Funded by: Clinical Medical Foundation of Chinese Medical Association
          Award ID: 18010330762
          Funded by: Suzhou Science and Technology Development Project
          Award ID: SYS201566
          Funded by: the Science and Technology Project of Changzhou
          Award ID: CJ20180040
          This work was supported by the Suzhou Key Medical Center(SZZX201505), Jiangsu Provincial Medical Innovation Team(CXTDB2017013), Suzhou Introduced Project of Clinical Medical Expert Team (SZYJTD201708), Suzhou Key Laboratory of male reproduction research (SZS201718), Suzhou Science and Technology Development Project (SYS201566), Clinical Medical Foundation of Chinese Medical Association (18010330762), Open Fund of State Key Laboratory of Reproductive Medicine of Nanjing Medical University (SKLRM-KA201704), Basic Program of Nanjing Medical University (2017NJMU163), Natural Science Foundation of Jiangsu Province (BK20160177 and BK20170562), the Science and Technology Project of Changzhou (CJ20180040), Matemity and Child Health Foundation of Jiangsu Province (F201865), National Natural Science Foundation of China (31701298, 81601338), Natural Science Foundation of the Jiangsu Higher Education Institutions of China (16KJB310006) and Key Research Foundation of Zhenjiang Social Development (SH2016028, SH2017013 and SH2018065)..
          Categories
          Research Paper

          PI3K/AKT signaling,germ cells,spermatogonial stem cell (SSC),Myotubularin related protein 7 (MTMR7)

          Comments

          Comment on this article