Blog
About

0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Heterogeneous Distribution of [ 3H]α,β-Methylene ATP Binding Sites in Blood Vessels

      ,

      Journal of Vascular Research

      S. Karger AG

      [3H]α, β-methylene ATP, ATP, Purinergic neurotransmission, Artery, Vein, Aorta, Purinoceptor

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          A radioligand binding assay and autoradiographic localisation of P2χ-purinoceptors were carried out in several different blood vessels from rat, guinea-pig and rabbit by using [<sup>3</sup>H]α, β-methylene ATP ([<sup>3</sup>H]α, β-MeATP) as the radioligand. The radioligand binding assay on rabbit ear artery showed that the binding process was saturable, and a high density of P<sub>2x</sub>-purinoceptors was observed. Autoradiographic localisation showed that the specific [<sup>3</sup>H]α, β-MeATP binding sites were only associated with the smooth muscle of the blood vessels. Semi-quantitation of the autoradiographs revealed significant differences in the densities of P<sub>2x</sub>-purinoceptors amongst the vessels studied. Generally the medium- and small-sized arteries had higher densities of P<sub>2x-</sub>purinoceptor than the elastic and large muscular arteries. In some large muscular arteries, such as the rabbit carotid, renal, hepatic and mesenteric, the outer region of vessel had a higher density of receptors than the inner region. The veins in this study were sparsely labelled, except portal veins where the longitudinal and circular layers of muscles were found to have different densities of P<sub>2x</sub>-purinoceptors. The results from this study provide direct evidence for the existence of P<sub>2x</sub>-purinoceptors in blood vessels; furthermore, the distribution of the P<sub>2x</sub>-purinoceptors is consistent with known pharmacological responses elicited by ATP in these vessels.

          Related collections

          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1993
          1993
          23 September 2008
          : 30
          : 2
          : 87-101
          Affiliations
          Department of Anatomy and Developmental Biology and Centre for Neuroscience, University College London, London, UK
          Article
          158980 J Vasc Res 1993;30:87–101
          10.1159/000158980
          8504200
          © 1993 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 15
          Categories
          Research Paper

          Comments

          Comment on this article