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      Heterogeneous Distribution of [ 3H]α,β-Methylene ATP Binding Sites in Blood Vessels


      Journal of Vascular Research

      S. Karger AG

      [3H]α, β-methylene ATP, ATP, Purinergic neurotransmission, Artery, Vein, Aorta, Purinoceptor

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          A radioligand binding assay and autoradiographic localisation of P2χ-purinoceptors were carried out in several different blood vessels from rat, guinea-pig and rabbit by using [<sup>3</sup>H]α, β-methylene ATP ([<sup>3</sup>H]α, β-MeATP) as the radioligand. The radioligand binding assay on rabbit ear artery showed that the binding process was saturable, and a high density of P<sub>2x</sub>-purinoceptors was observed. Autoradiographic localisation showed that the specific [<sup>3</sup>H]α, β-MeATP binding sites were only associated with the smooth muscle of the blood vessels. Semi-quantitation of the autoradiographs revealed significant differences in the densities of P<sub>2x</sub>-purinoceptors amongst the vessels studied. Generally the medium- and small-sized arteries had higher densities of P<sub>2x-</sub>purinoceptor than the elastic and large muscular arteries. In some large muscular arteries, such as the rabbit carotid, renal, hepatic and mesenteric, the outer region of vessel had a higher density of receptors than the inner region. The veins in this study were sparsely labelled, except portal veins where the longitudinal and circular layers of muscles were found to have different densities of P<sub>2x</sub>-purinoceptors. The results from this study provide direct evidence for the existence of P<sub>2x</sub>-purinoceptors in blood vessels; furthermore, the distribution of the P<sub>2x</sub>-purinoceptors is consistent with known pharmacological responses elicited by ATP in these vessels.

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          Author and article information

          J Vasc Res
          Journal of Vascular Research
          S. Karger AG
          23 September 2008
          : 30
          : 2
          : 87-101
          Department of Anatomy and Developmental Biology and Centre for Neuroscience, University College London, London, UK
          158980 J Vasc Res 1993;30:87–101
          © 1993 S. Karger AG, Basel

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          Page count
          Pages: 15
          Research Paper


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