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      Evidence for anticipation in Beckwith–Wiedemann syndrome

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          Abstract

          Classical Beckwith–Wiedemann syndrome (BWS) was diagnosed in two sisters and their male cousin. The children's mothers and a third sister were tall statured (178, 185 and 187 cm) and one had mild BWS features as a child. Their parents had average heights of 173 cm (mother) and 180 cm (father). This second generation tall stature and third generation BWS correlated with increased methylation of the maternal H19/IGF2-locus. The results were obtained by bisulphite treatment and subclone Sanger sequencing or next generation sequencing to quantitate the degree of CpG-methylation on three locations: the H19 promoter region and two CTCF binding sites in the H19 imprinting control region (ICR1), specifically in ICR1 repeats B1 and B7. Upon ICR1 copy number analysis and sequencing, the same maternal point variant NCBI36:11:g.1979595T>C that had been described previously as a cause of BWS in three brothers, was found. As expected, this point variant was on the paternal allele in the non-affected grandmother. This nucleotide variant has been shown to affect OCTamer-binding transcription factor-4 (OCT4) binding, which may be necessary for maintaining the unmethylated state of the maternal allele. Our data extend these findings by showing that the OCT4 binding site mutation caused incomplete switching from paternal to maternal ICR1 methylation imprint, and that upon further maternal transmission, methylation of the incompletely demethylated variant ICR1 allele was further increased. This suggests that maternal and paternal ICR1 alleles are treated differentially in the female germline, and only the paternal allele appears to be capable of demethylation.

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          Beckwith-Wiedemann syndrome.

          Beckwith-Wiedemann syndrome (BWS) is a model disorder for the study of imprinting, growth dysregulation, and tumorigenesis. Unique observations in this disorder point to an important embryonic developmental window relevant to the observations of increased monozygotic twinning and an increased rate of epigenetic errors after subfertility/assisted reproduction.
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            Beckwith-Wiedemann syndrome.

            Beckwith-Wiedemann syndrome (BWS) is an imprinting disorder characterized by overgrowth, tumor predisposition, and congenital malformations. Approximately 85% of reported BWS cases are sporadic, while the remaining 15% are familial. BWS is caused by epigenetic or genomic alterations which disrupt genes in one or both of the two imprinted domains on chromosome 11p15.5. In each domain, an imprinting center regulates the expression of imprinted genes in cis. Normally in domain 1, insulin-like growth factor 2 (IGF2) and the untranslated mRNA H19 are monoallelically expressed. In BWS, increased expression of IGF2 occurs via several mechanisms. In domain 2, CDKN1C, a growth repressor, and an untranslated RNA, KCNQ1OT1, are normally expressed monoallelically. In cases of BWS, several mechanisms result in reduced expression of CDKN1C. Recent reports of BWS cases have identified mutations outside the chromosome 11p15.5 critical region, thereby broadening the challenges in the diagnosis and genetic counseling of individuals and families with BWS.
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              Protection against De Novo Methylation Is Instrumental in Maintaining Parent-of-Origin Methylation Inherited from the Gametes

              Introduction Fertilization ensures the propagation of genetic and epigenetic information from one generation to the next. In mammals, epigenetic and long-lasting effects inherited in a parent-of-origin manner are known as genomic imprinting (Barlow, 2011). The main epigenetic mark that ensures their transmission and effects is DNA methylation. Methylation marks at imprinted loci are established in a sex-specific manner during gametogenesis, at genomic loci referred to as germline differentially methylated regions (gDMRs). After fertilization, these gDMRs act in cis to control the monoallelic and parent-specific expression of a subset of genes, the imprinted genes. Germline DMRs can affect imprinted expression in a variety of ways, including promoter control of protein-coding and noncoding RNAs, regulation of transcription elongation, and long distance insulator activities. The allelic differences of gDMRs also include a typical chromatin signature, consisting of both repressive and permissive histone marks (McEwen and Ferguson-Smith, 2010). Disruption of imprinted expression upon genetic deletion of a gDMR is ultimate proof that it functions as an imprinting control region (ICR). To date, around 120 imprinted genes have been identified in mouse and human. They are under the control of 20 identified gDMRs/ICRs, 17 of which are methylated in the oocyte (maternal gDMRs), and only three in sperm (paternal gDMRs). Parental ICRs are also sexually dimorphic in terms of CpG content and genomic localization: maternal ICRs are CpG island (CGI) promoters, while paternal ICRs are relatively CpG poor and intergenic. Evolutionary reasons for these discrepancies may be linked to the different developmental kinetics of male and female gametogenesis (Bourc’his and Bestor, 2006; Schulz et al., 2010). Importantly, maternal ICRs have a dominant role in early development, regulating biological pathways related to the establishment of the feto-maternal interface (Schulz et al., 2010). It is becoming increasingly clear that the acquisition of sex-specific methylation patterns extends beyond imprinted regions in gametes (Kobayashi et al., 2012a; Smallwood et al., 2011; Smith et al., 2012). CpG islands are more prone to being methylated in the oocyte than in sperm and globally, about a thousand CGIs may be specifically methylated in the oocyte genome, exceeding the number of known maternal ICRs by far. Imprinted and nonimprinted methylation is likely to be established in the same way in the oocyte, under the control of the de novo DNA methyltransferase DNMT3A and its cofactor DNMT3L, and in a transcription-dependent manner (Bourc’his et al., 2001; Chotalia et al., 2009; Kaneda et al., 2004; Kobayashi et al., 2012a; Smallwood et al., 2011). Instead of specific targeting mechanisms for their establishment in gametes, what truly distinguishes ICRs from the rest of the genome is their treatment after fertilization, in the wake of the extensive methylation changes inherent to early mammalian development. Before implantation, methylated alleles of ICRs are resistant to the genome-wide erasure of gametic methylation that coincides with the acquisition of embryonic pluripotency. Specific trans acting factors have been identified as critical in maintaining ICR methylation during this period, such as binding of the KRAB (Krüppel-associated box-containing) zinc finger protein system, which involves Zfp57 and the heterochromatin inducer KAP1/TRIM28 (Li et al., 2008; Mackay et al., 2008; Quenneville et al., 2011; Zuo et al., 2012). Following this critical period, parental differences in ICR methylation are thought to persist throughout life, with no stage- and tissue-specificity, although this aspect of imprinting has not been fully addressed. To gain insight into the extent of gametic methylation inheritance, we performed a genome-wide screen for gDMRs in the mouse postimplantation embryo. Considering their larger number, we specifically looked for maternally transmitted gDMRs, by comparative methylation profiling of wild-type embryos and embryos lacking oocyte-inherited methylation, using MeDIP-Seq (Methylated DNA Immunoprecipitation followed by high throughput sequencing). We exploited the well-characterized Dnmt3L mutant system, in which postimplantation Dnmt3L-/+ embryos generated by fertilization of Dnmt3L−/− oocytes completely lack maternal imprints, while methylation patterns at paternal ICRs and repeats are unaltered (Bourc’his et al., 2001; Schulz et al., 2010). Our approach proved to be highly sensitive and efficient at identifying regions of oocyte-inherited methylation. We found all 17 known maternal gDMRs/ICRs and less than thirty new candidates, revealing that loci that maintain maternal-specific methylation are rare in the postimplantation embryo. We characterized two new maternally imprinted gDMRs, located within the Cdh15 gene and at the promoter of AK008011, a retroposed pseudogene. Further analyses enabled us to demonstrate that inherited maternal gDMRs can exist in a permanent or transient state after fertilization, and that avoidance of de novo methylation during embryo or tissue differentiation plays a key role in the permanency of parent-of-origin methylation inherited from the gametes. Results A Genome-wide Screen for Regions of Oocyte-Inherited Methylation by MeDIP-Seq We generated DNA methylation profiles by MeDIP-Seq for pools of 8.5 dpc (days post coïtum) embryos of either a wild-type or Dnmt3L-/+ genotype. For each pool, two independent MeDIP preparations were sequenced. We obtained 41 M and 32.7 M distinct and uniquely alignable reads for wild-type and Dnmt3L-/+ embryos, respectively (Supplemental Information). For a 1000 bp sliding window size and a permissive false discovery rate (FDR) threshold of  0.5 (Schulz et al., 2010). Given this fact and due to the functional link between CG density and DNA methylation-mediated transcriptional control (Weber et al., 2007), we excluded DMR candidates that contained fewer than 10 CpGs and that had a ratio of  25%. These filters reduced the DMR number to 96, 47 hypomethylated and 49 hypermethylated, which showed a dispersed distribution throughout the mouse genome (Figure 1D). Improved Definition of Known Imprinted Loci and Identification of New gDMRs We first used our MeDIP-Seq data to improve the genetic map of germline or secondary DMRs for known imprinted loci that have not been fully documented. Two hypomethylated DMRs coincided with the promoters of the Slc38a4 (FDR < 0.5) and Peg12 (FDR < 0.05) genes (Figures S1E and S1F), and were confirmed to be hypomethylated in Dnmt3L-/+ embryos by MSRE-qPCR assays (Methylation-Sensitive Restriction Enzyme coupled with quantitative PCR) (Figure 1E). Paternal-specific expression and maternal-specific methylation had been previously reported at these loci in somatic tissues (Kobayashi et al., 2002; Smith et al., 2003). Reduced-Representation Bisulfite Sequencing (RRBS) confirmed here that the Slc38a4 DMR is indeed methylated in oocyte and hypomethylated in sperm and can be categorized as a genuine maternal gDMR (Figure S3A). However, the Peg12 DMR was unmethylated in both oocyte and sperm, suggesting that it is not a gDMR. Our screen also led to a reassessment of the imprinted Gpr1-Zdbf2 locus, which was originally characterized as a fourth imprinted region controlled by paternal methylation (Hiura et al., 2010; Kobayashi et al., 2009). We identified two hypermethylated DMRs (FDR < 0.01 and 0.07), which overlap with two originally described paternal gDMRs (DMR2 and DMR3) (Figures 1A and S1G). However, as discussed earlier, paternal gDMRs, such as the H19-Igf2 DMR, are unaltered in our screen. The methylation status of the Gpr1-Zdbf2 DMRs in Dnmt3L-/+ embryos is instead reminiscent of a secondary, somatic DMR similar to the Nesp DMR, as validated by MSRE-qPCR (Figure 1E) and bisulfite sequencing (data not shown), and was recently independently confirmed (Kobayashi et al., 2012b). In our search for additional novel maternal gDMRs, we focused on 28 hypomethylated, single-copy and relatively CpG-rich candidate DMRs (Table 1), which importantly do not belong to known imprinted regions. All but three of the candidate gDMRs were within a transcription unit, among which ten were located to an annotated promoter, six overlapped with the last exon/3′ UTR, and nine were within a gene body (Table S1 and Figure 1F). Contrary to known maternal ICRs, all of which coincide with promoter-associated CGIs, only nine out of 28 candidates overlapped with a CGI and only five of those were associated with an annotated promoter. To evaluate which candidate gDMRs may constitute regions of bona fide oocyte-specific methylation, we interrogated publically available CGI methylation data in the mouse oocyte (Kobayashi et al., 2012a): all but two CGI candidates from our screen were found methylated. We further integrated MeDIP-Seq data from mouse C57Bl6/J sperm (Table 1), and found only one of the candidates to be methylated in sperm, confirming that sequences methylated in the oocyte are usually not methylated in sperm (Kobayashi et al., 2012a; Smallwood et al., 2011; Smith et al., 2012). As an indication of long-term maintenance of maternal-specific methylation, we integrated MeDIP-Seq data from fetal 17.5 dpc hybrid mouse liver from C57Bl6/J and PWD/PhJ strain crosses. In these samples, parental allele-specific sequencing reads were counted at known SNPs between the parental strains (Supplemental Information). For six candidate gDMRs, we found evidence for maternal-specific methylation maintenance (binomial p < 0.2) (Table 1). Of note, the lack of evidence for maternal-specific methylation from liver was mostly due to a paucity of SNPs (7/28 DMRs) or low read depth over an existing SNP (14/28 DMRs). Using bisulfite sequencing, we further assessed four of these candidates. None of these showed maternal-specific methylation in fetal liver (Figure S4), confirming that very few new maternal gDMRs persisting after implantation are left to be uncovered. We went on to study four candidate maternal gDMRs in more detail, chosen for their high level of significance in our screen and their association with a CGI, a systematic feature of currently known maternal ICRs. Importantly, MSRE-qPCR and bisulfite sequencing confimed their hypomethylation in 8.5 dpc Dnmt3L-/+ embryos and in sperm (Figure 1E and data not shown). Three are located in gene bodies toward the 3′ end of the respective canonical RefSeq transcript (Cdh15, Zfp777 and Zfp787). The fourth candidate overlaps with the promoter of AK008011, a mono-exonic retrogene. The Cdh15 DMR Controls the Paternal- and Tissue-Specific Expression of an Intragenic Transcript The Cdh15 DMR (ranked 14th; FDR < 0.02) spans exons 10 to 12 of the Cdh15 gene (Figures 1A and 2A), which maps to distal chromosome 8 (8qE2) and encodes the M-cadherin protein, a cell-adhesion protein linked to muscle and cerebellum (Padilla et al., 1998; Rose et al., 1995). By bisulfite sequencing of exon 11, we showed that this DMR fulfills the three developmental criteria of a maternally imprinted gDMR (Figures 2B and S3B): (1) methylation acquisition in the oocyte but not in sperm, (2) maintenance of maternally methylated alleles prior to implantation, as revealed by the lack of methylated alleles in maternal-imprint free Dnmt3L-/+ blastocysts compared to wild-type blastocysts, and (3) protection of the paternally unmethylated alleles after implantation, as shown in 9.5 dpc embryos derived from C57Bl6/J and CAST/Ei strains. Moreover, we demonstrated that the maternal allele is unable to regain methylation in Dnmt3L-/+ postimplantation embryos, confirming the obligate passage through the female germline to imprint this locus. In adult tissues and cells derived from C57Bl6/J by CAST/Ei or 129 Sv by CAST/Ei crosses, the methylated status of maternal alleles was consistently maintained. In quadriceps, tail and hypothalamus, methylation differences between parental alleles were highly significant (Fisher’s exact p < 10−11), although a minority of paternal alleles tended to regain methylation in quadriceps (Figure 2C). MeDIPSeq analysis of fetal hybrid liver DNA also showed higher methylation of maternal compared to paternal alleles (binomial p = 0.133) (Table 1). However, in ES cells, adult cortex and cerebellum, parental specificity was lost due to acquisition of methylation on the paternal alleles (Figure 2D). The intragenic Cdh15 DMR is therefore conserved during adulthood, but in a tissue-specific manner. We next investigated the chromatin state of the Cdh15 DMR by immunoprecipitation (ChIP). We measured the quantity and allelic specificity of three marks associated with imprinted DMRs (H3K4me2, H3K9me2 and H4K20me3), in MEFs (mouse embryonic fibroblasts), which globally maintain maternal-specific DNA methylation (Figure 3A). The Cdh15 DMR was found enriched in repressive H3K9me2 and H4K20me3, at a level similar to the typical maternal ICR KvDMR (Figure 3B). Permissive H3K4me2 marks were also found at this locus. We assayed the allele-specificity of these marks by pyrosequencing, exploiting SNPs between the C57Bl6/J and CAST/Ei strains (Figure 3C). H3K4me2 was associated with the paternal allele, while H4K20me3 was enriched on the maternal allele. In contrast to KvDMR, for which H3K9me2 is maternally enriched, this mark was equally distributed on both parental alleles at the Cdh15 DMR. This shows that the Cdh15 DMR harbors opposite allelic states of histone modifications in MEFs, with respect to H3K4 and H4K20 methylation. Cdh15 is highly expressed in satellite cells of skeletal muscles and granular cells of the cerebellum (Cornelison and Wold, 1997; Rose et al., 1995). Moreover, evidence for paternal-specific expression was recently reported in adult hypothalamus (Gregg et al., 2010). By using quantitative RT-PCR, we readily detected Cdh15 expression in quadriceps, cerebellum and hypothalamus, with the strongest detection in cerebellum (Figure 3D). Expression measurements were equal upstream and downstream of the DMR (exon 1 versus exons 12–13) for the quadriceps and the cerebellum, suggesting the existence of a transcript elongating from the 5′ canonical promoter throughout the coding unit. However, in the hypothalamus, 10-fold greater expression was measured downstream of the DMR, suggesting the existence of a transcript originating intragenically. Northern blot analysis confirmed the production of a single full-length transcript in the cerebellum, around the expected 3 kb size, while the hypothalamus specifically expressed a shorter version of Cdh15, which could be detected with a probe spanning exons 9–14 (Figure 3E), but not exons 5–9 (data not shown). Both transcripts were present in neonatal brains. The allelic status of Cdh15 expression was determined in reciprocal BxC and CxB crosses: while biallelic expression was found upstream and downstream of the DMR in quadriceps and cerebellum, only paternal expression of the short Cdh15 transcript was detected in hypothalamus (Figure 3F). In neonatal brains, a switch from biallelic to monoallelic expression was observed at the DMR. Further allelic mapping by RT-PCR revealed that the short paternal transcript originates between exons 9 and 10, 5′ of the Cdh15 DMR, a region that showed maternal-specific methylation in neonatal brain (Figures S5A and S5B). The Cdh15 DMR probably corresponds to an intragenic promoter, specifically active in brain cell-types detectable at birth and in the hypothalamus at adulthood. Its differential methylation correlates with differential allelic transcription in these cell types. The Cdh15 DMR defines chromosome 8 as a new chromosome harboring an imprinted locus. To determine the extent of Cdh15 DMR control, we measured the allelic expression of the three closest neighboring genes (Acsf3, AK040202 and Ankrd11), by an RT-PCR pyrosequencing-based approach. We did not detect imprinted expression for these genes, in a bank of reciprocal hybrid tissues including embryonic, fetal, neonate and adult stages (Figure S6). It is therefore highly probable that the Cdh15 DMR does not control an imprinted cluster. Of clinical interest, the human CDH15 gene has been associated with intellectual disability (Bhalla et al., 2008). The CDH15 gene has a similar genomic organization to its mouse homolog, notably with an intragenic CGI spanning exons 9 to 12. We analyzed the imprinted status of this locus in human fetal liver, a tissue we find to maintain maternal-specific methylation in mouse. Unexpectedly, the 5′ part of the CGI was completely methylated, while the 3′ part that includes the region homologous to the sequence we analyzed in mouse was completely unmethylated (Figure S5C). Lack of methylation was confirmed in lymphocyte and placental DNA (data not shown). Our study does not support a conservation of imprinting for the CDH15 locus in humans but rather points to a bipartite CGI. The AK008011 DMR Is a Tissue-Specific Imprinted gDMR at a Mouse Pseudogene The second DMR we focused on maps to a CGI located 5′ of AK008011, an intronless gene (Figure 4A). It was likely generated via the retrotransposition of a Coro1c mRNA (Coronin, Actin binding protein 1c located on chromosome 5) to a region 1.5 kb downstream of Nhlrc1 on chromosome 13qA5, an event that occurred specifically in the mouse lineage (Kent et al., 2003). We uncovered this small DMR through a 500 bp sliding window analysis (FDR < 0.35) (Table S1), while no DMR was identified at the Coro1c locus. Bisulfite-based methylation analysis revealed 1) methylation acquisition in oocyte but not in sperm, 2) protection of maternally methylated alleles prior to implantation and 3) protection of paternally unmethylated alleles after implantation (Figure 4B). This locus therefore behaves as a typical maternal imprinted gDMR during the critical window around fertilization and implantation. However, in adult life, this gDMR becomes tissue-specific. While maternal-specific methylation is properly maintained in tail and fibroblasts (Figure 4C), the quadriceps, cortex and liver show dense methylation (over 60%) of both paternal and maternal alleles (Figure 4D). This finding again questions the view of the permanency of imprinted gDMRs throughout life. In an attempt to investigate the impact of this gDMR on allelic expression, we designed primers that specifically distinguish AK008011 from Coro1c mRNA. However, we could not detect expression in tissues where the gDMR is conserved. The high rate of nucleotide divergence between mouse strains suggests a low selective pressure on this gene, which may be a pseudogene: 40 SNPs are referenced at AK008011, including 9 nonsynonymous ones, versus 6 synonymous changes at the transcribed region of Coro1c (MGI and dbSNP build 128). Further examination of the closest gene, Nhlrc1, did not reveal a bias in parental expression in any tissue from our hybrid bank (data not shown), suggesting that the AK008011 gDMR does not have long-range imprinting effects. Zfp777 and Zfp787 DMRs Are Transient Maternal gDMRs The last two hypomethylated DMRs we validated (FDR < 2%) map to CGIs located in the last exon of the Zfp777 (6qB2.3) and Zfp787 (7qA1) genes, which encode zinc finger proteins (Figures 5A and S7A). As is typical for maternal gDMRs, we found methylation acquisition specifically in the oocyte, and protection of maternally methylated alleles prior to implantation (Figures 5B, S3B, and S7B). However, paternal alleles of these DMRs undergo de novo methylation at implantation, so that both parental alleles displayed similar levels of methylation at 9.5 dpc as well as in 17.5 dpc fetal liver (Table 1). Contrary to imprinting-associated gDMRs, which show lifelong parental differences at least in some tissues, these DMRs may be categorized as transient gDMRs. Interestingly, in Dnmt3L-/+ embryos, paternal and maternal alleles were equally methylated at 9.5 dpc, suggesting no differential treatment of the two alleles (Figures 5B and S7B). The global methylation per parental allele was slightly lower than age-matched wild-type embryos, likely as a consequence of a postimplantation developmental delay (Bourc’his et al., 2001). Examination of various normal adult tissues by bisulfite sequencing confirmed complete methylation later in life (Figures 5C and S7C). Further analysis of the last exon of ZNF777 in human postimplantation tissues confirmed the existence of methylated alleles only (data not shown). The observation of parent-specific marks at the blastocyst stage prompted us to analyze the allelic methylation of these transient gDMRs in ES cells. Bisulfite analysis showed that ES cells do not reproduce the parental methylation differences of their biological progenitors; the Zfp777 DMR was biallelically methylated, while the Zfp787 DMR was biallelically hypomethylated (Figures 5D and S7C). This was observed in ES cells that were isolated and grown in conditions optimal for “ground-state” pluripotency cells (2i medium) (Nichols et al., 2009), and in ES cells cultured in classical medium (data not shown). Similar to previous findings (Borgel et al., 2010; Dean et al., 1998), our results demonstrate that ES cells do not necessarily maintain the allelic status of sequences that are differentially methylated in the blastocyst, and specifically, may not be a suitable cellular model for studying transient gDMRs. When allelic expression patterns were measured in hybrid reciprocal tissues, we found no parental bias in Zfp777 and Zfp787 expression, even in the preimplantation blastocyst, where parental methylation differences still exist (Figures 5E and S7D). Our results suggest that the methylation located in the 3′ end of the Zfp777 and Zfp787 genes may not functionally impinge on their expression. Discussion In mammals, the oocyte and sperm genomes harbor distinct methylation patterns, as a result of different kinetics and constraints exerted on gamete production in the two sexes. The inheritance of parent-specific methylation at fertilization provides the opportunity for differential allelic regulation in the progeny, with genomic imprinting as the most durable form of parent-specific regulation of gene expression. Our present work demonstrates that the total number of maternal germline DMRs persisting throughout development and adulthood is very limited, in line with current estimates for the number of known ICRs. From this study, it can be concluded that genomic imprinting is an unusual form of regulation in mammals. Recent genome-wide studies have highlighted preimplantation demethylation as a major determinant of gametic methylation clearance (Borgel et al., 2010; Kobayashi et al., 2012a; Smallwood et al., 2011). We reveal here that de novo methylation plays an equally important role in leveling parental methylation differences inherited from the gametes (Figure 6). Zfp777 and Zfp787 DMRs lose their maternal specificity early, by paternal methylation acquisition at implantation. Cdh15 and AK008011 DMRs are protected at implantation, but nevertheless gain paternal methylation later, during tissue differentiation. The permanency and universality of imprinted gDMRs was a commonly held notion in genomic imprinting. The tissue-specificity of Cdh15 and AK008011 gDMRs revisits this notion and highlights the limitation of studies performed on a specific adult tissue for identifying new imprinted gDMRs. In this regard, a recent genome-wide screen performed on adult mouse cortex uncovered nine candidate regions of parent-of-origin methylation (Xie et al., 2012), of which only two candidates overlapped with our unfiltered candidate list (AK008011 and Casc1). Interestingly, loss of parent-specific marks by de novo methylation may not be restricted to the new imprinted loci we describe: indeed, at traditionally known ICRs, loss of differential methylation has been sporadically reported in normal adult cells, occurring by methylation gain, rather than loss (Fang et al., 2012; Ferrón et al., 2011). Our work increases the number of known imprinted gDMRs to 23, including two new loci to be referenced. While the Cdh15 DMR is associated with parent-specific expression, the AK008011 DMR may not be functional, showing that imprinted gDMRs may not necessarily be selected for a role in gene regulation. Previous studies had alluded to a possible imprinted status of Cdh15. Analysis of chromosome 8 duplications led to the characterization of a region of complete maternal methylation and intermediate paternal methylation in embryos and neonates (Kelsey et al., 1999). While no parent-specific Cdh15 expression was found in embryos, a recent analysis reported paternal-specific expression of three SNPs confined to exons 12 to 14, in adult hypothalamus (Gregg et al., 2010). Our study resolves the modus operandi of this locus, by the identification of a maternal gDMR that maps to Cdh15 exons 10–12, which is maintained in a tissue-specific manner and controls the paternal expression of a short alternative transcript in neonatal brain and adult hypothalamus. The Cdh15 DMR may be a docking site for transcription factors expressed in specific brain cell types, whose binding/activity is impaired by maternal DNA methylation. Cdh15/M-cadherin is an adhesion protein that mediates cell-to-cell interactions. Homozygous Cdh15 null mice are viable, and show no apparent defects in skeletal muscle and cerebellum, likely due to compensation from other cadherins (Hollnagel et al., 2002). Moreover, there is no evidence of parent-of-origin effects in these mutant mice. However, the corresponding deletion targets exons 1 to 4, and therefore, should not impair the production of the short imprinted Cdh15 transcript. Interestingly, a similar 5′ truncated form of cadherin with altered adhesion activity has been described in specific neurons of the chick embryo (Shirabe et al., 2005). Provided that the short imprinted Cdh15 transcript is translated, it may likewise exert specific functions in mammalian hypothalamic cells, related to cell communication, polarization and shaping. By identifying both imprinted and transient gDMRs, our screen highlights that these two types of gDMRs are indistinguishable in gametes and early embryos (Figure 6). Recruitment of KAP1 through Zfp57 binding was shown to be required for the maintenance of methylated alleles of ICRs (Li et al., 2008; Mackay et al., 2008; Quenneville et al., 2011; Zuo et al., 2012). By in silico analysis we found that all the maternal gDMRs we validated (Slc38a4, Cdh15, AK008011, Zfp777 and Zfp787) contain some hexanucleotide motifs for Zfp57 binding (Table S1). Moreover, Zfp57 and KAP1 are enriched at these sites in published ES cell ChIP-Seq data (Table S1) (Quenneville et al., 2011). Our study therefore shows that the presence of Zfp57 motifs cannot be used as a hallmark of genomic imprinting, as it is also found at transient gDMRs. However, it is likely to specify all genomic sequences that maintain gametic methylation during preimplantation development. Interestingly, four Zfp57 binding motifs exist at the intragenic CGI of the human CDH15 locus. While we found no evidence of imprinting, we cannot exclude that this locus is a true maternal gDMR in human, either transient or tissue-specific. Methylation gain at implantation is what discriminates transient from lifelong imprinted gDMRs. The former are permissive, while the latter are refractory to this process. Zfp777, Zfp787, and Cdh15 DMRs are all intragenic sequences, for which a strong positive correlation has been reported between DNA methylation and transcriptional read through from the host gene (Ball et al., 2009; Chotalia et al., 2009). Paternal de novo methylation at transient Zfp777 and Zfp787 gDMRs may therefore be facilitated by ongoing transcription from these genes at implantation. Conversely, at the imprinted Cdh15 gDMR, low levels of transcription from the main upstream promoter, local enrichment in H3K4 methylation and transcription factor occupancy may protect from de novo methylation (Lienert et al., 2011; Ooi et al., 2007). The same rules would apply later during life with tissue formation. The DMR is conserved in tissues where it acts as an active promoter for the short paternal Cdh15 transcript (hypothalamus and neonatal brain), and is potentially protected by transcription factors and/or H3K4me marks. However, in tissues where the short transcript is not expressed, different methylation states are observed and seem to correlate with the expression status of the long canonical Cdh15 transcript. Our screen designed at 8.5 dpc was effective at identifying tissue-specific imprinted gDMRs, because it was performed at a time when they are still universal. It also identified transient gDMRs during their remethylation process. Although the transient gDMRs we found do not seem to affect expression, presumably because of their 3′ position, other transient gDMRs may regulate the transcriptome of the peri-implantation embryo. Notably, the parental specificity of these methylated sequences should be lost upon somatic nuclear transfer, resulting in two methylated alleles instead of one during preimplantation development. Furthermore, as for imprinted gDMRs, transient gDMRs may be sensitive to assisted reproductive technologies, involving stimulation of oocyte production and preimplantation embryo culture. Experimental Procedures MeDIP-Seq MeDIP-Seq was performed on three pooled litters with the Dnmt3L+/+ (WT) or Dnmt3L-/+ genotype. All MeDIP and sequencing library preparations were performed in parallel. Additionally, MeDIP-Seq was performed on three independent C57Bl6/J sperm samples and twelve pools of three livers each of 17.5 dpc fetal hybrid C57Bl6/J and PWD/PhJ mice. MeDIP enrichment and preparation of paired-end sequencing libraries were then performed as described (Down et al., 2008), using monoclonal anti-5-methylcytosine antibody (Eurogentech) and magnetic anti-mouse beads (Dynabeads) for immunoprecipitation. All libraries were sequenced using an Illumina GA2x instrument. DNA Methylation Analyses For MSRE-qPCR, the methylation-dependent restriction enzyme McrBC was used, and methylation percentages were calculated according to (Oakes et al., 2009). Values represent the average of three independent digestion experiments, performed on DNA from 8.5 dpc litters of eight embryos. For bisulfite conversion, DNA was treated with the EpiTect kit (QIAGEN). BiQ Analyzer software was used for sequence alignments (Bock et al., 2005) and clones with identical patterns of conversion were removed from the final pileup.
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                Author and article information

                Journal
                Eur J Hum Genet
                Eur. J. Hum. Genet
                European Journal of Human Genetics
                Nature Publishing Group
                1018-4813
                1476-5438
                December 2013
                10 April 2013
                1 December 2013
                : 21
                : 12
                : 1344-1348
                Affiliations
                [1 ]Center for Medical Genetics and Molecular Medicine, Haukeland University Hospital , Bergen, Norway
                [2 ]Department of Clinical medicine, University of Bergen , Bergen, Norway
                [3 ]Institut für Humangenetik, Universitätsklinikum Essen , Essen, Germany
                [4 ]Faculty of Medicine, University of Southampton , Southampton, UK
                [5 ]Wessex Genetics Service, Southampton University Hospitals Trust , Southampton, UK
                [6 ]Salisbury Hospital NHS Foundation Trust , Salisbury, UK
                Author notes
                [* ]Center for Medical Genetics and Molecular Medicine Haukeland University Hospital , Jonas Liesvei N-5021, Bergen, Norway. Tel: +47 55 97 54 44; Fax: +47 55 97 54 78; E-mail: gunnar.houge@ 123456helse-bergen.no
                Article
                ejhg201371
                10.1038/ejhg.2013.71
                3831082
                23572028
                fa3ddb53-be41-4cef-b615-8d9e72fa5b89
                Copyright © 2013 Macmillan Publishers Limited

                This work is licensed under a Creative Commons Attribution 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/3.0/

                History
                : 03 October 2012
                : 02 March 2013
                : 12 March 2013
                Categories
                Article

                Genetics
                igf2,beckwith–wiedemann syndrome,imprinting,h19,anticipation
                Genetics
                igf2, beckwith–wiedemann syndrome, imprinting, h19, anticipation

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