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      miR-138 inhibits gastric cancer growth by suppressing SOX4.

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          Abstract

          MicroRNA-138 (miR-138) has been reported to be downregulated and function as a tumor suppressor in several cancers. However, the role and molecular mechanisms of miR-138 in the progression of gastric cancer (GC) remain to be clarified. The aim of the present study was to determine the role of miR-138 in GC progression. In the present study we found that miR-138 expression was downregulated in GC tissues and cell lines. Statistical analysis demonstrated that low expression levels of miR-138 were associated with advanced tumor-node-metastasis (TNM) stage, and lymph node metastasis. Function assays demonstrated that overexpression of miR-138 impaired GC cell proliferation, colony formation, migration and invasion in vitro, as well as suppressed tumor growth in vivo. Through reporter gene, qRT-PCR and western blot assays, SRY-related high mobility group box 4 (SOX4), a master mediator in epithelial-mesenchymal transition (EMT), was confirmed to be a direct target of miR-138 in GC cells. Western blot assay revealed that miR-138 overexpression inhibited EMT procession in GC cells by upregulation of epithelial marker E-cadherin and downregulation of mesenchymal markers, N-cadherin and vimentin. Furthermore, the levels of miR-138 were inversely correlated with those of SOX4 expression in GC tissues. Overexpression of SOX4 rescued the inhibition effect in GC cells caused by miR-138. Collectively, these findings indicate that miR-138 may be a potential therapeutic target for GC.

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          Author and article information

          Journal
          Oncol. Rep.
          Oncology reports
          Spandidos Publications
          1791-2431
          1021-335X
          Aug 2017
          : 38
          : 2
          Affiliations
          [1 ] Department of Anesthesiology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
          [2 ] Department of Colorectal and Anal Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
          [3 ] Institute of Virology and AIDS Research, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
          [4 ] Operating Room, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.
          Article
          10.3892/or.2017.5745
          28656304

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