33
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: not found
      • Article: not found

      Evaluation effects of chitosan for the extracellular matrix production by fibroblasts and the growth factors production by macrophages

      , , , , ,
      Biomaterials
      Elsevier BV

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Chitosan is reported as an accelerator of wound healing. Histological findings of previous reports indicate that chitosan accelerates the reformation of connective tissue, however the details of the mechanism are not clear. In this study, firstly L929 mouse fibroblasts were cultured with chitosan and the production of extracellular matrix (ECM) was evaluated in vitro. Type I and III collagens and fibronectin were secreted by L929 with or without chitosan; however there was no significant difference in the amount of ECM between the control and the chitosan groups. Secondly, macrophages were stimulated with chitosan, and then transforming growth factor-beta 1 (TGF-beta1) and platelet-derived growth factor (PDGF) messenger ribonucleic acid (mRNA) expressions and production of their proteins were assayed in vitro. As a result, chitosan promoted the production of TGF-beta1 and PDGF. These results indicate that chitosan does not directly accelerate ECM production by fibroblast and the ECM production may increase by the growth factors.

          Related collections

          Author and article information

          Journal
          Biomaterials
          Biomaterials
          Elsevier BV
          01429612
          August 2001
          August 2001
          : 22
          : 15
          : 2125-2130
          Article
          10.1016/S0142-9612(00)00401-4
          11432592
          fa9acbc1-8a18-4d3c-ae99-c497273bc80b
          © 2001

          https://www.elsevier.com/tdm/userlicense/1.0/

          History

          Comments

          Comment on this article