19
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Corrigendum to “The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs” [Genes & Diseases 5 (2018) 62–74]

      correction

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          The authors regret having an image assembly error in Figure 5Ca, in which the image for the “0h dBiFP-AdRFP” group was erroneously duplicated with an overlapping image from the “36h BiFP dBIFP-AdR-simH19” group. We confirm the error is restricted to the image assembly, and the underlying data and conclusions are correct and unchanged. The authors would like to apologize for any inconvenience caused. Image 1 Figure 5 The highly degradable dBiFP as a sensitive transcription reporter of lncRNA H19. ( A )Schematic representation of the construction of dBiFP-H19 transcriptional reporter. Mouse lncRNA H19 (mH19) was cloned into the downstream of the stop codon of the dBiFP coding region. It is expected a chimeric transcript of dBiFP-mH19 will be generated, which will be further translated into the highly degradable protein dBiFP. Targeting mH19 by siRNAs, miRNAs, naturally occurring or synthetic modulatory RNAs, or small molecule compounds may lead to a decrease in the chimeric transcript and thus the decrease in BiFP signal. As a proof-of-principle experiment, we use an adenoviral vector expressing mH19-specific siRNAs, AdR-simH19 to knockdown mH19 transcript. ( B ) Generation of a stable dBiFP-H19 reporter line from HCT116 cells. TqPCR analysis indicates the high expression of mouse H19 in the dBiFP-H19 HCT116 stable line (p < 0.01 compared with that of the control line dBiFP). ( C ) H19-specific siRNAs effectively down-regulate dBiFP expression in dBiFP-H19 cells. Subconfluent dBiFP and dBiFP-H19 cells were infected with AdR-simH19 or AdRFP. Fluorescence signals were recorded at 0h and 36h after infection ( a ). The BiFP fluorescence signal was quantitatively analyzed ( b ). “∗∗“, P < 0.01 compared the AdR-simH19 infection with that of AdRFP infection. ( D ) TqPCR analysis of mouse H19 in dBiFP-H19 cells upon AdR-simH19 silencing. Subconfluent dBiFP and dBiFP-H19 cells were infected with AdR-simH19 or AdRFP for 36h. Total RNA was isolated and subjected to RT-PCR analysis. TqPCR assay was done in triplicate. “∗∗” P < 0.01 when compared the expression level of AdR-simH19 infection with that of AdRFP infection

          Related collections

          Author and article information

          Contributors
          Journal
          Genes Dis
          Genes Dis
          Genes & Diseases
          Chongqing Medical University
          2352-4820
          2352-3042
          26 February 2023
          March 2023
          26 February 2023
          : 10
          : 2
          : 627-629
          Affiliations
          [a ]Ministry of Education Key Laboratory of Diagnostic Medicine and School of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, China
          [b ]Molecular Oncology Laboratory, Department of Orthopaedic Surgery and Rehabilitation Medicine, The University of Chicago Medical Center, Chicago, IL 60637, USA
          [c ]Department of Clinical Laboratory Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, China
          [d ]Departments of Clinical Laboratory Medicine, General Surgery, Orthopedic Surgery, Nephrology, and Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
          [e ]The Children's Hospital, Chongqing Medical University, Chongqing 400014, China
          [f ]Department of Laboratory Medicine and Clinical Diagnostics, The Affiliated Yantai Hospital, Binzhou Medical University, Yantai 264100, China
          [g ]Department of Immunology and Microbiology, Beijing University of Chinese Medicine, Beijing 100029, China
          [h ]Key Laboratory of Orthopaedic Surgery of Gansu Province and the Department of Orthopaedic Surgery, The Second Hospital of Lanzhou University, Lanzhou 730030, China
          [i ]Department of Surgery, The Affiliated Zhongnan Hospital of Wuhan University, Wuhan 430071, China
          [j ]Department of Biochemistry and Molecular Biology, China Three Gorges University School of Medicine, Yichang 443002, China
          [k ]Department of Orthopaedic Surgery, Chongqing Hospital of Traditional Chinese Medicine, Chongqing 400021, China
          [l ]Department of Orthopaedic Surgery, Xiangya Second Hospital of Central South University, Changsha 410011, China
          [m ]Chongqing Key Laboratory for Oral Diseases and Biomedical Sciences, Chongqing 401147, China
          [n ]The Affiliated Hospital of Stomatology of Chongqing Medical University, Chongqing 401147, China
          [o ]Department of Surgery, Laboratory of Craniofacial Biology and Development, Section of Plastic Surgery, The University of Chicago Medical Center, Chicago, IL 60637, USA
          Author notes
          []Corresponding author. tche@ 123456uchicago.edu
          [∗∗ ]Corresponding author. wuxingye19830221@ 123456126.com
          Article
          S2352-3042(23)00025-9
          10.1016/j.gendis.2023.02.004
          10201670
          fa9fbade-e27e-421f-a238-f784e0e2c905
          © 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

          This is an open access article under the CC BY-NC-SA license (http://creativecommons.org/licenses/by-nc-sa/4.0/).

          History
          Categories
          Corrigenda

          Comments

          Comment on this article