The current work provides a revised and updated key to the genera of Eucharitidae occurring in the New World, with the major traits of genera illustrated through high-resolution photomicrography. We provide an annotated list of genera and species currently known from Brazil, with a new record for a genus previously unknown for the country. Additionally, we provide a literature revision of the biology and cuticular hydrocarbon profile for the Eucharitidae. The lack of knowledge on the eucharitid Brazilian fauna is briefly discussed.