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      Superresolution optical fluctuation imaging (SOFI).

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          Abstract

          Superresolution microscopy has shifted the limits for fluorescence microscopy in cell -biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation -imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.

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          Author and article information

          Journal
          Adv. Exp. Med. Biol.
          Advances in experimental medicine and biology
          Springer Nature
          0065-2598
          0065-2598
          2012
          : 733
          Affiliations
          [1 ] Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA, USA. thomasd@chem.ucla.edu
          Article
          10.1007/978-94-007-2555-3_2
          22101708

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