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Amino acid analysis: Determination of cysteine plus half-cystine in proteins after hydrochloric acid hydrolysis with a disulfide compound as additive
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Abstract
All cysteine and cystine in a protein are derivatized during hydrolysis in hydrochloric
acid containing 3,3'-dithiodipropionic acid. The resulting derivative can be separated
from other amino acids and used for quantitation of cysteine plus half-cystine. A
procedure is presented for accurate determination by ion exchange chromatography and
postcolumn derivatization of all amino acids from acid hydrolysis of a protein, including
the Cys-derivative.