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      Sealing ability of three root-end filling materials prepared using an erbium: Yttrium aluminium garnet laser and endosonic tip evaluated by confocal laser scanning microscopy

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          Abstract

          Aims:

          (1) To compare the sealing ability of mineral trioxide aggregate (MTA), Biodentine, and Chitra-calcium phosphate cement (CPC) when used as root-end filling, evaluated under confocal laser scanning microscope using Rhodamine B dye. (2) To evaluate effect of ultrasonic retroprep tip and an erbium:yttrium aluminium garnet (Er:YAG) laser on the integrity of three different root-end filling materials.

          Materials and Methods:

          The root canals of 80 extracted teeth were instrumented and obturated with gutta-percha. The apical 3 mm of each tooth was resected and 3 mm root-end preparation was made using ultrasonic tip ( n = 30) and Er:YAG laser ( n = 30). MTA, Biodentine, and Chitra-CPC were used to restore 10 teeth each. The samples were coated with varnish and after drying, they were immersed in Rhodamine B dye for 24 h. The teeth were then rinsed, sectioned longitudinally, and observed under confocal laser scanning microscope.

          Statistical Analysis Used:

          Data were analyzed using one-way analysis of variance (ANOVA) and a post-hoc Tukey's test at P < 0.05 (R software version 3.1.0).

          Results:

          Comparison of microleakage showed maximum peak value of 0.45 mm for Biodentine, 0.85 mm for MTA, and 1.05 mm for Chitra-CPC. The amount of dye penetration was found to be lesser in root ends prepared using Er:YAG laser when compared with ultrasonics, the difference was found to be statistically significant ( P < 0.05).

          Conclusions:

          Root-end cavities prepared with Er:YAG laser and restored with Biodentine showed superior sealing ability compared to those prepared with ultrasonics.

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          Most cited references19

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          Mineral trioxide aggregate: a review of the constituents and biological properties of the material.

          This paper reviews the literature on the constituents and biocompatibility of mineral trioxide aggregate (MTA). A Medline search was conducted. The first publication on the material was in November 1993. The Medline search identified 206 papers published from November 1993 to August 2005. Specific searches on constituents and biocompatibility of mineral trioxide aggregate, however, yielded few publications. Initially all abstracts were read to identify which fitted one of the two categories required for this review, constituents or biocompatibility. Based on this assessment and a review of the papers, 13 were included in the constituent category and 53 in the biocompatibility category. Relatively few articles addressed the constituents of MTA, whilst cytological evaluation was the most widely used biocompatibility test.
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            Root-end filling materials: apical microleakage and marginal adaptation.

            The purpose of this study was to evaluate the root-end sealing ability through dye leakage evaluation and the marginal adaptation through scanning electron microscopy (SEM) of some root-end filling materials. Thirty human uniradicular teeth were used. Teeth were divided into three groups: (1) retrofilled with MTA-Angelus, (2) with SuperEBA, and (3) with Vitremer. The root surfaces were isolated with nail polish and teeth were immersed in silver nitrate. Roots were sectioned transversally at each millimeter, in three sections and evaluated at a stereomicroscope to observe dye penetration. Using SEM the distance between the tested root-end filling materials and the surrounding dentin was measured at four points. The statistical analysis showed significant differences among the three materials in relation to the sealing ability (p < 0.05). Concerning marginal adaptation, MTA-Angelus presented the best results (p < 0.01). Absence of correlation between the two methodologies was clearly observed.
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              “Evaluation of sealing ability of MM-MTA, Endosequence, and biodentine as furcation repair materials: UV spectrophotometric analysis”

              Aim: To evaluate the sealing ability of MICRO-MEGA Mineral Trioxide Aggregate, Endosequence, Biodentine as furcation repair materials using a dye extraction leakage method. Materials and Methods: Forty mandibular molars were randomly divided according to the material used for perforation repair. Group I- (left unsealed) control, Group II-MICRO-MEGA Mineral Trioxide Aggregate, Group III - Endosequence, Group IV - Biodentine. All samples were subjected to orthograde and retrograde methylene blue dye challenge followed by dye extraction with 65% nitric acid. Samples were then analyzed using Ultra violet (UV) Visible Spectrophotometer. Results: Biodentine showed highest dye absorbance, whereas Endosequence showed lowest dye absorbance when compared with other repair materials. Conclusion: Within the limitations of this study, it was observed that Endosequence showed better sealing ability when compared with other root repair materials.
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                Author and article information

                Journal
                J Conserv Dent
                J Conserv Dent
                JCD
                Journal of Conservative Dentistry : JCD
                Medknow Publications & Media Pvt Ltd (India )
                0972-0707
                0974-5203
                Jul-Aug 2015
                : 18
                : 4
                : 327-330
                Affiliations
                [1]Department of Conservative Dentistry and Endodontics, Coorg Institute of Dental Science, Virajpet, Karnataka, India
                Author notes
                Address for correspondence: Dr. Salin Nanjappa Ammanda, Department of Conservative Dentistry and Endodontics, Coorg Institute of Dental Science, Virajpet - 571 218, Karnataka, India. E-mail: salinnanjappa@ 123456gmail.com
                Article
                JCD-18-327
                10.4103/0972-0707.159746
                4502131
                26180420
                fb664f6b-6ee5-4b3c-8a3e-4e6426a3b226
                Copyright: © Journal of Conservative Dentistry

                This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

                History
                : 22 January 2015
                : 15 March 2015
                : 07 April 2015
                Categories
                Original Article

                Dentistry
                biodentine,chitra-cpc,confocal laser scanning microscope,er:yag laser,mta
                Dentistry
                biodentine, chitra-cpc, confocal laser scanning microscope, er:yag laser, mta

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