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      Effects of acteoside on lipopolysaccharide-induced inflammation in acute lung injury via regulation of NF-κB pathway in vivo and in vitro.

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          Abstract

          The purpose of the present study was to investigate the protective role of acteoside (AC) on lipopolysaccharide (LPS)-induced acute lung injury (ALI). BalB/c mice intraperitoneally received AC (30, and 60 mg/kg) or dexamethasone (2 mg/kg) 2h prior to or after intratracheal instillation of LPS. Treatment with AC significantly decreased lung wet-to-dry weight (W/D) ratio and lung myeloperoxidase (MPO) activity and ameliorated LPS-induced lung histopathological changes. In addition, AC increased super oxide dismutase (SOD) level and inhibited malondialdehyde (MDA) content, total cell and neutrophil infiltrations, and levels of proinflammatory cytokines including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) in bronchoalveolar lavage fluid (BALF) in LPS-stimulated mice. Furthermore, we demonstrated that AC inhibited the phosphorylation of IκBα, nuclear factor-κB (NF-κB) p65, inhibitor of nuclear factor kappa-B kinase-α (IKK-α) and inhibitor of nuclear factor kappa-B kinase-β (IKKβ) in LPS-induced inflammation in A549 cells. Our data suggested that LPS evoked the inflammatory response in lung epithelial cells A549. The experimental results indicated that the protective mechanism of AC might be attributed partly to the inhibition of proinflammatory cytokine production and NF-κB activation.

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          Author and article information

          Journal
          Toxicol. Appl. Pharmacol.
          Toxicology and applied pharmacology
          1096-0333
          0041-008X
          Jun 1 2015
          : 285
          : 2
          Affiliations
          [1 ] Changchun University of Chinese Medicine, Changchun 130117, China.
          [2 ] Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: machunhuabest@126.com.
          [3 ] Changchun University of Chinese Medicine, Changchun 130117, China. Electronic address: wangshuminch@126.com.
          Article
          S0041-008X(15)00124-6
          10.1016/j.taap.2015.04.004
          25902336
          fb872a7b-5946-4834-9b9d-2be71eef8c2e
          Copyright © 2015 Elsevier Inc. All rights reserved.

          Acteoside,Lipopolysaccharide (LPS),Lung epithelial cells,Lung injury,Mice,Nuclear factor-κB (NF-κB)

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