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      Semen cryopreservation: Successes and persistent problems in farm species

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      Canadian Journal of Animal Science

      Agricultural Institute of Canada

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          Abstract

          Artificial insemination has arguably been the most important practice contributing to the advancement of animal production. The numerous advantages of artificial insemination are augmented when the semen is cryopreserved and conserved for long periods. Unfortunately, the utility of cryopreserved semen is limited because for most mammals, even cattle, a considerable proportion of sperm loose their fertility during freezing-thawing. In many species , this loss of fertility is substantial, rendering cryopreserved semen impractical for routine use. Our goals are to characterize the nature of the sublethal sperm damage caused by cryopreservation, and to develop a method to improve the functional fertility of thawed semen. This review describes our theory that sperm are prematurely activated by cryopreservation (“cryo-capacitation”) and discusses strategies for preventing this damage. Key words: Sperm, artificial insemination, capacitation, fertility

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          Author and article information

          Journal
          Canadian Journal of Animal Science
          Can. J. Anim. Sci.
          Agricultural Institute of Canada
          0008-3984
          1918-1825
          September 01 2003
          September 01 2003
          : 83
          : 3
          : 393-401
          Article
          10.4141/A03-024
          © 2003
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