Harnessing sustainable nanoclusters for sensitive optical detection of tetracyclines and the underlying mechanism†

Abstract

Simple and rapid sensing methods for the detection of antibiotics in drinks and foods are highly desirable due to the presence of these drugs in food products, as a consequence of extensive abuse of antibiotics in livestock production. In this study, we report a facile synthesis strategy of gold nanoclusters (AuNCs) passivated with N-acetyl- l-cysteine (AuNC@NAC). This nanocluster exhibits a fluorescence emission peak at 700 nm, which gradually decreases at increasing concentrations of antibiotics, such as tetracyclines. The limit of detection (LOD) was determined to be 0.8 ppm with a linear range of 0.1–140 μM (0.04–62 ppm). This method showcased exceptional selectivity in the detection of tetracyclines compared to anions, metallic cations and amino acids. The underlying mechanism has been elucidated, and the fluorescence quenching was found to be a combination of dynamic and static quenching mechanisms, with photoinduced electron transfer (PET) identified as the primary process for dynamic quenching.

Abstract

N-Acetyl- l-cysteine-capped gold nanoclusters obtained by an easy green approach show high sensitivity and selectivity in detecting tetracyclines over a wide range. Fluorescence quenching primarily involves photoinduced electron transfer.